PMID- 21953953
OWN - NLM
STAT- MEDLINE
DCOM- 20120106
LR  - 20151119
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 25
IP  - 20
DP  - 2011 Oct 30
TI  - The role of liquid chromatography and flow injection analyses coupled to isotope 
      ratio mass spectrometry for studying human in vivo glucose metabolism.
PG  - 2989-94
LID - 10.1002/rcm.5179 [doi]
AB  - Under most physiological conditions, glucose, or carbohydrate (CHO), homeostasis 
      is tightly regulated. In order to mechanistically appraise the origin of 
      circulating glucose (e.g. via either gluconeogenesis, glycogenolysis or oral 
      glucose intake), and its regulation and oxidation, the use of stable isotope 
      tracers is now a well-accepted analytical technique. Methodologically, liquid 
      chromatography coupled to isotope ratio mass spectrometry (LC/IRMS) can replace 
      gas chromatography coupled to combustion-isotope ratio mass spectrometry 
      (GC/C/IRMS) for carrying out compound-specific (13)C isotopic analysis. The 
      LC/IRMS approach is well suited for studying glucose metabolism, since the plasma 
      glucose concentration is relatively high and the glucose can readily undergo 
      chromatography in an aqueous mobile phase. Herewith, we report two main 
      methodological approaches in a single instrument: (1) the ability to measure the 
      isotopic enrichment of plasma glucose to assess the efficacy of CHO-based 
      treatment (cocoa-enriched) during cycling exercise with healthy subjects, and (2) 
      the capacity to carry out bulk isotopic analysis of labeled solutions, which is 
      generally performed with an elemental analyzer coupled to IRMS. For plasma 
      samples measured by LC/IRMS the data show a isotopic precision SD(delta(13)C) and 
      SD(APE) of 0.7  per thousand and 0.001, respectively, with delta(13)C and APE values of -25.48  per thousand 
      and 0.06, respectively, being generated before and after tracer administration. 
      For bulk isotopic measurements, the data show that the presence of organic 
      compounds in the blank slightly affects the delta(13)C values. Despite some 
      analytical limitations, we clearly demonstrate the usefulness of the LC/IRMS 
      especially when (13)C-glucose is required during whole-body human nutritional 
      studies.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Godin, Jean-Philippe
AU  - Godin JP
AD  - Nestle Research Center, Nestec, Ltd., Lausanne, Switzerland. 
      jean-philippe.godin@rdls.nestle.com
FAU - Stellingwerff, Trent
AU  - Stellingwerff T
FAU - Actis-Goretta, Lucas
AU  - Actis-Goretta L
FAU - Mermoud, Anne-France
AU  - Mermoud AF
FAU - Kochhar, Sunil
AU  - Kochhar S
FAU - Rezzi, Serge
AU  - Rezzi S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Blood Glucose)
RN  - 0 (Carbon Isotopes)
RN  - AR09D82C7G (Deuterium)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Blood Glucose/*metabolism
MH  - Breath Tests
MH  - Carbon Isotopes
MH  - Chromatography, Liquid/*methods
MH  - Deuterium
MH  - Exercise
MH  - Flow Injection Analysis/*methods
MH  - Glucose/administration & dosage/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Mass Spectrometry/*methods
MH  - Reproducibility of Results
EDAT- 2011/09/29 06:00
MHDA- 2012/01/10 06:00
CRDT- 2011/09/29 06:00
PHST- 2011/09/29 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
AID - 10.1002/rcm.5179 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2011 Oct 30;25(20):2989-94. doi: 10.1002/rcm.5179.