PMID- 21954834
OWN - NLM
STAT- MEDLINE
DCOM- 20120423
LR  - 20211203
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 14
IP  - 1
DP  - 2012 Jan
TI  - Enhancement of the immunosuppressive effect of human adipose tissue-derived 
      mesenchymal stromal cells through HLA-G1 expression.
PG  - 70-9
LID - 10.3109/14653249.2011.613926 [doi]
AB  - BACKGROUND AIMS: Mesenchymal stromal cells (MSC) from several tissues have 
      immunomodulatory properties that involve various immunosuppressive molecules. An 
      example is human leukocyte antigen (HLA)-G, a non-classical major 
      histocompatibility complex (MHC) class I molecule that induces tolerance via 
      interactions with inhibitory receptors present on major immune effector cells. 
      Recently, the molecular mechanisms that regulate MSC-mediated immunosuppression 
      have come under investigation. Our goal was to determine whether HLA-G plays a 
      crucial role in immunosuppression and whether human adipose tissure (hAT) MSC can 
      be used as a tool for biologic immunosuppression with HLA-G in transplantation. 
      METHODS: MSC were characterized by fluorescence-activated cell sorting (FACS) 
      analysis, reverse transcriptase (RT)-polymerase chain reaction (PCR) and staining 
      for differentiation. The immunogenicity and immunomodulatory effects of MSC were 
      monitored by peripheral blood mononuclear cell (PBMC) proliferation assay with or 
      without phytohemagglutinin (PHA) stimulation. Stable expression of HLA-G1 in MSC 
      was done using a lentiviral system. Results. MSC from different tissues had 
      similar morphology, immunophenotypic characters and differentiation potential. We 
      also found that the immunosuppressive effect of MSC was monitored along with 
      their endogenous HLA-G mRNA and protein levels. Stable expression of HLA-G1 
      appeared to enhance the immunosuppressive effect of hAT MSC, and the function of 
      HLA-G1 was significantly decreased by HLA-G antagonistic antibody in PBMC 
      proliferation assays. CONCLUSIONS: Although the HLA-G molecule is not the sole 
      factor for MSC-mediated immunosuppression, our data provide evidence that HLA-G 
      plays an important role in immunosuppression and that hAT MSC can be used as a 
      tool for biologic immunosuppression during transplantation procedures.
FAU - Yang, Heung-Mo
AU  - Yang HM
AD  - Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of 
      Medicine, Seoul, Republic of Korea.
FAU - Sung, Ji-Hye
AU  - Sung JH
FAU - Choi, Young-Sil
AU  - Choi YS
FAU - Lee, Hyun-Joo
AU  - Lee HJ
FAU - Roh, Cheong-Rae
AU  - Roh CR
FAU - Kim, Jongman
AU  - Kim J
FAU - Shin, Milljae
AU  - Shin M
FAU - Song, Sanghyun
AU  - Song S
FAU - Kwon, Choon-Hyuck
AU  - Kwon CH
FAU - Joh, Jae-Won
AU  - Joh JW
FAU - Kim, Sung-Joo
AU  - Kim SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110928
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Antibodies, Blocking)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Receptors, Fc)
SB  - IM
MH  - Adipose Tissue/cytology/immunology
MH  - Antibodies, Blocking/pharmacology
MH  - Cell Differentiation/drug effects/genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Gene Expression Regulation, Developmental/drug effects/genetics
MH  - HLA-G Antigens/genetics/*metabolism
MH  - Humans
MH  - Immune Tolerance/drug effects/genetics
MH  - *Immunosuppression Therapy
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/drug effects/*immunology
MH  - *Organ Transplantation
MH  - Receptors, Fc/immunology
MH  - Transgenes/genetics
EDAT- 2011/10/01 06:00
MHDA- 2012/04/24 06:00
CRDT- 2011/09/30 06:00
PHST- 2011/09/30 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/04/24 06:00 [medline]
AID - S1465-3249(12)70616-3 [pii]
AID - 10.3109/14653249.2011.613926 [doi]
PST - ppublish
SO  - Cytotherapy. 2012 Jan;14(1):70-9. doi: 10.3109/14653249.2011.613926. Epub 2011 
      Sep 28.