PMID- 21955507
OWN - NLM
STAT- MEDLINE
DCOM- 20120207
LR  - 20111024
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 89
DP  - 2012 Jan 1
TI  - Improving immobilization of lipase onto magnetic microspheres with moderate 
      hydrophobicity/hydrophilicity.
PG  - 73-8
LID - 10.1016/j.colsurfb.2011.08.031 [doi]
AB  - Magnetic microspheres with carboxyl groups were prepared by copolymerization of 
      vinyl acetate (VAC), acrylamide (AM), and acrylic acid (AA) in the presence of 
      oleic acid-coated Fe(3)O(4) nanoparticles. Scanning electron microscope (SEM) 
      photo showed that the average diameter of magnetic microspheres was about 400 nm. 
      Also, FTIR spectra analysis indicated that monomers were successfully enfolded on 
      the microspheres' surface. They were used as support to immobilize lipase via 
      physical adsorption and covalent binding. To investigate the effect of the 
      microsphere surface properties on lipase immobilization, a series of microspheres 
      with different hydrophobic/hydrophilic surface characteristics were prepared by 
      adjusting molar percentages of different monomers. The results showed that 
      microspheres with different hydrophobicities/hydrophilicities had different 
      immobilized ratios and different activity recovery. Compared with microspheres 
      having hydrophilic characteristics, that with hydrophobic characteristics had a 
      much higher lipase binding efficiency. However, this study further demonstrated 
      that moderate hydrophobicity/hydrophilicity of microsphere surface was very 
      important for elevating activity recovery. When AM (hydrophilic monomer) held 
      14.3% of total amount of monomers, the activity recovery was the highest 
      (reaching 87%, 418 U/g support). Possible reasons for these observations were 
      discussed and a supposed mechanism was speculated.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Zhang, Dong-Hao
AU  - Zhang DH
AD  - College of Pharmacy, Hebei University, Baoding, China. zhangdonghao1@gmail.com
FAU - Yuwen, Li-Xia
AU  - Yuwen LX
FAU - Xie, Yu-Lei
AU  - Xie YL
FAU - Li, Wei
AU  - Li W
FAU - Li, Xiao-Bing
AU  - Li XB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110914
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Enzymes, Immobilized)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Adsorption
MH  - Enzymes, Immobilized/*metabolism
MH  - Lipase/*metabolism
MH  - *Magnetics
MH  - *Microspheres
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2011/10/01 06:00
MHDA- 2012/02/09 06:00
CRDT- 2011/09/30 06:00
PHST- 2011/06/24 00:00 [received]
PHST- 2011/08/26 00:00 [revised]
PHST- 2011/08/29 00:00 [accepted]
PHST- 2011/09/30 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/02/09 06:00 [medline]
AID - S0927-7765(11)00519-4 [pii]
AID - 10.1016/j.colsurfb.2011.08.031 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2012 Jan 1;89:73-8. doi: 
      10.1016/j.colsurfb.2011.08.031. Epub 2011 Sep 14.