PMID- 21957157
OWN - NLM
STAT- MEDLINE
DCOM- 20120214
LR  - 20230604
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 301
IP  - 6
DP  - 2011 Dec
TI  - Impact of a single session of intermittent pneumatic leg compressions on skeletal 
      muscle and isolated artery gene expression in rats.
PG  - R1658-68
LID - 10.1152/ajpregu.00457.2011 [doi]
AB  - Intermittent pneumatic leg compressions (IPC) have proven to be an effective 
      noninvasive approach for treatment of patients with claudication, but the 
      mechanisms underlying the clinical benefits remain elusive. In the present study, 
      a rodent model of claudication produced by bilateral ligation of the femoral 
      artery was used to investigate the acute impact of a single session of IPC (150 
      min) on hemodynamics, skeletal muscle (tibialis anterior), and isolated 
      collateral artery (perforating artery) expression of a subset of genes associated 
      with inflammation and vascular remodeling. In addition, the effect of compression 
      frequency (15 vs. 3 compressions/min) on the expression of these factors was 
      studied. In ligated animals, IPC evoked an increase of monocyte chemoattractant 
      protein-1 (MCP-1) and cytokine-induced neutrophil chemoattractant 1 (CXCL1) mRNA 
      (P < 0.01) and immunostaining (P < 0.05), as well as a minor increase in VEGF 
      immunostaining in the muscle endomysium 150 min postintervention. Further, 
      collateral arteries from these animals showed an increased expression of MCP-1 
      (approximately twofold, P = 0.02). These effects were most evident in the group 
      exposed to the high-frequency protocol (15 compressions/min). In contrast, IPC in 
      sham-operated control animals evoked a modest initial upregulation of VEGF (P = 
      0.01), MCP-1 (P = 0.02), and CXCL1 (P = 0.03) mRNA in the muscle without 
      concomitant changes in protein levels. No changes in gene expression were 
      observed in arteries isolated from sham animals. In conclusion, IPC acutely 
      up-regulates the expression of important factors involved in vascular remodeling 
      in the compressed muscle and collateral arteries in a model of hindlimb ischemia. 
      These effects appear to be dependent on the compression frequency, such that a 
      high compression frequency (15 compressions/min) evokes more consistent and 
      robust effects compared with the frequency commonly employed clinically to treat 
      patients with claudication (3 compressions/min).
FAU - Roseguini, Bruno T
AU  - Roseguini BT
AD  - Department of Biomedical Sciences, University of Missouri, Columbia, Missouri 
      65211, USA.
FAU - Arce-Esquivel, Arturo A
AU  - Arce-Esquivel AA
FAU - Newcomer, Sean C
AU  - Newcomer SC
FAU - Laughlin, M H
AU  - Laughlin MH
LA  - eng
GR  - R01 HL036088/HL/NHLBI NIH HHS/United States
GR  - HL-36088/HL/NHLBI NIH HHS/United States
GR  - RR-18276/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110928
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Cxcl1 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Arteries/*metabolism
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemokine CXCL1/genetics/*metabolism
MH  - Gene Expression Regulation/physiology
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - *Pressure
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
PMC - PMC3233847
EDAT- 2011/10/01 06:00
MHDA- 2012/02/15 06:00
PMCR- 2012/12/01
CRDT- 2011/09/30 06:00
PHST- 2011/09/30 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/02/15 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - ajpregu.00457.2011 [pii]
AID - R-00457-2011 [pii]
AID - 10.1152/ajpregu.00457.2011 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2011 Dec;301(6):R1658-68. doi: 
      10.1152/ajpregu.00457.2011. Epub 2011 Sep 28.