PMID- 21958002
OWN - NLM
STAT- MEDLINE
DCOM- 20120223
LR  - 20111021
IS  - 1557-8577 (Electronic)
IS  - 1549-1684 (Linking)
VI  - 14
IP  - 5
DP  - 2011 Oct
TI  - Effect of growth hormone treatment on pancreatic inflammation, oxidative stress, 
      and apoptosis related to aging in SAMP8 mice.
PG  - 501-12
LID - 10.1089/rej.2011.1166 [doi]
AB  - Aging is associated with an increase in inflammation, oxidative stress, and 
      apoptosis. Furthermore, aging is accompanied by an alteration of the growth 
      hormone (GH) -insulin-like growth factor-1 (IGF-1) axis. The aim of this study 
      was to examine the regulation of these parameters in the pancreas of old mice and 
      how GH treatment could affect this process. Male senescence-accelerated prone 
      mice (SAMP8) and male senescence-accelerated resistant mice (SAMR1) 2 (young) and 
      10 months old were used (n = 40). Animals were divided into five experimental 
      groups: 1 and 2, SAMP8/R1 young control; 3 and 4, SAMP8/R1 old control 
      (untreated); and 5, SAMP8 old treated with GH. Physiologically equivalent doses 
      of GH were administered for 1 month (2 mg subcutaneously [s.c.]/kg/day) and 
      several parameters were analyzed. Aging was associated with increased 
      inflammation, oxidative stress, and apoptosis (increased tumor necrosis factor-alpha 
      [TNF-alpha], interleukin-beta [IL-beta], IL-6, monocyte chemoattractant protein-1 [MCP1], 
      IL-2, heme oxygenase [HO-1], inducible nitric oxide synthase [iNOS], and nitric 
      oxide metabolites [NOx]). The ratio of anti/pro apoptotic mRNA expression-B cell 
      lymphoma 2 (Bcl-2) Bcl-2-associated X protein (BAX) + Bcl-xL/Bcl-2-associated 
      death promoter (BAD)-was decreased during aging in SAMP8 mice. X-inhibitor of 
      apoptosis (XIAP) was decreased during the aging process. Furthermore, no changes 
      were observed in protein expression of nuclear factor-kappaB (NF-kappaB p65 and 
      NF-kappaBp50-105. However, the protein expression of NF-kappaB p52-100 and inhibitor 
      kappa B (IkappaB) alpha was increased with age in the pancreas of SAMP8 mice. On the 
      other hand, the expression of IkappaB beta was decreased with aging. These results 
      indicate that aging is associated with significant alterations in the relative 
      expression of pancreatic genes involved in inflammation, oxidative stress, and 
      apoptosis. According to our results, GH administration to old SAMP8 mice was able 
      to improve pancreas from this parameters.
FAU - Cuesta, Sara
AU  - Cuesta S
AD  - Department Physiology, Medical School, University Complutense of Madrid Medical 
      School, University Complutense of Madrid, Madrid, Spain.
FAU - Kireev, Roman
AU  - Kireev R
FAU - Garcia, Cruz
AU  - Garcia C
FAU - Forman, Katherine
AU  - Forman K
FAU - Vara, Elena
AU  - Vara E
FAU - Tresguerres, Jesus A F
AU  - Tresguerres JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110929
PL  - United States
TA  - Rejuvenation Res
JT  - Rejuvenation research
JID - 101213381
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (X-Linked Inhibitor of Apoptosis Protein)
RN  - 0 (bcl-Associated Death Protein)
RN  - 0 (bcl-X Protein)
RN  - 12629-01-5 (Human Growth Hormone)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Aging/*drug effects/pathology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Human Growth Hormone/administration & dosage/*pharmacology
MH  - I-kappa B Proteins/metabolism
MH  - Inflammation/genetics/*pathology
MH  - Interleukin-1beta/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Mutant Strains
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Pancreas/drug effects/metabolism/*pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - X-Linked Inhibitor of Apoptosis Protein/genetics/metabolism
MH  - bcl-Associated Death Protein/genetics/metabolism
MH  - bcl-X Protein/genetics/metabolism
EDAT- 2011/10/01 06:00
MHDA- 2012/02/24 06:00
CRDT- 2011/10/01 06:00
PHST- 2011/10/01 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/02/24 06:00 [medline]
AID - 10.1089/rej.2011.1166 [doi]
PST - ppublish
SO  - Rejuvenation Res. 2011 Oct;14(5):501-12. doi: 10.1089/rej.2011.1166. Epub 2011 
      Sep 29.