PMID- 21958362
OWN - NLM
STAT- MEDLINE
DCOM- 20120720
LR  - 20171116
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 47
IP  - 2
DP  - 2012 Apr
TI  - Areca nut extracts suppress the differentiation and functionality of human 
      monocyte-derived dendritic cells.
PG  - 198-203
LID - 10.1111/j.1600-0765.2011.01421.x [doi]
AB  - BACKGROUND AND OBJECTIVE: Areca quid chewing, a major risk factor contributing to 
      the occurrence of oral cancer and precancer, has been reported to be associated 
      with the severity and high prevalence of periodontal diseases in areca quid 
      chewers. As dendritic cells are critically involved in the regulation of innate 
      and adaptive immunity in oral mucosa, the objective of the present study was to 
      investigate the effect of areca nut extracts (ANE) on the differentiation and 
      reactivity of dendritic cells derived from monocytes. MATERIAL AND METHODS: Human 
      peripheral blood monocytes were cultured in the presence of granulocyte-monocyte 
      colony-stimulating factor and interleukin-4 for 7 d to generate dendritic cells. 
      To examine the effect of ANE on the generation of dendritic cells, the monocytes 
      were exposed to ANE throughout the 7 d culture period. In addition, the effect of 
      ANE on the maturation of monocyte-derived dendritic cells induced by 
      lipopolysaccharide (LPS) was examined. RESULTS: Monocytes cultured in 
      granulocyte-monocyte colony-stimulating factor and interleukin-4 exhibited a 
      typical phenotype of dendritic cells, as evidenced by the heightened expression 
      of human leukocyte antigen (HLA)-DR, CD11c and the co-stimulatory molecules CD40, 
      CD80 and CD86. Exposure of the monocytes to ANE did not influence the expression 
      of HLA-DR and CD11c, but markedly attenuated the proportion of CD40-positive 
      cells and the mean fluorescence intensity of CD86. The expression of 
      co-stimulatory molecules in LPS-activated dendritic cells was not affected, 
      whereas the mRNA expression of interleukin-12 induced by LPS was markedly 
      suppressed by ANE treatment in a concentration-dependent manner. CONCLUSION: 
      These results suggest that ANE exposure interfered with the differentiation of 
      dendritic cells from monocytes. Moreover, the functionality of mature 
      monocyte-derived dendritic cells was attenuated in the presence of ANE.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Wang, C-C
AU  - Wang CC
AD  - School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
FAU - Chen, T-Y
AU  - Chen TY
FAU - Wu, H-Y
AU  - Wu HY
FAU - Liu, T-Y
AU  - Liu TY
FAU - Jan, T-R
AU  - Jan TR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110930
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Coloring Agents)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - *Areca
MH  - B7-1 Antigen/analysis
MH  - B7-2 Antigen/analysis
MH  - CD11c Antigen/analysis
MH  - CD40 Antigens/analysis
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects
MH  - Coloring Agents
MH  - Dendritic Cells/*drug effects/immunology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Interleukin-12/analysis
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/*drug effects/immunology
MH  - *Nuts
MH  - Phenotype
MH  - Plant Extracts/*pharmacology/toxicity
MH  - Tetrazolium Salts
MH  - Thiazoles
EDAT- 2011/10/01 06:00
MHDA- 2012/07/21 06:00
CRDT- 2011/10/01 06:00
PHST- 2011/10/01 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/07/21 06:00 [medline]
AID - 10.1111/j.1600-0765.2011.01421.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2012 Apr;47(2):198-203. doi: 10.1111/j.1600-0765.2011.01421.x. 
      Epub 2011 Sep 30.