PMID- 21959450
OWN - NLM
STAT- MEDLINE
DCOM- 20111229
LR  - 20220410
IS  - 1536-3732 (Electronic)
IS  - 1049-2275 (Linking)
VI  - 22
IP  - 5
DP  - 2011 Sep
TI  - Evaluation of hybrid porous biomimetic nano-hydroxyapatite/polyamide 6 and bone 
      marrow-derived stem cell construct in repair of calvarial critical size defect.
PG  - 1852-8
LID - 10.1097/SCS.0b013e31822e8274 [doi]
AB  - Nano-hydroxyapatite/polyamide 6 (n-HA/PA6) composite scaffolds were prepared by a 
      combined method of phase separation and particle leaching processing technique. 
      The porous matrices were seeded with allogenic bone marrow-derived stem cells 
      (BMSCs) and implanted in the critical-size calvarial defect (8-mm diameter) in 
      rats. The adhesion, proliferation, and differentiation of BMSCs were demonstrated 
      by (3-4,5-dimethylthiazol-2yl-2,5-diphenyl-2H-tetrazoliumbromide) assay, 
      immunohistochemical staining, and scanning electron microscopy in vitro. Cell 
      survival was tracked via fluorescent labeling in vivo. The regeneration process 
      was evaluated by several techniques at 4, 8, and 16 weeks after implantation. 
      According to the results, the porous n-HA/PA6 scaffolds are biocompatible and 
      have no negative effects on the BMSCs in vitro. Nano-hydroxyapatite/polyamide 6 
      composite combined with BMSCs exhibited good biocompatibility and 
      osteoconductivity and enhanced osteogenesis at the initial stage after 
      implantation. The results demonstrated that porous n-HA/PA6 scaffold is a 
      potential matrix for bone tissue engineering, and using BMSCs as seed cells on 
      porous n-HA/PA6 might be an alternative procedure in repair of large defect due 
      to enhanced osteogenesis and osteointegration.
FAU - Khadka, Ashish
AU  - Khadka A
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, 
      People's Republic of China.
FAU - Li, Jihua
AU  - Li J
FAU - Li, Yubao
AU  - Li Y
FAU - Gao, Ying
AU  - Gao Y
FAU - Zuo, Yi
AU  - Zuo Y
FAU - Ma, Yongqing
AU  - Ma Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Craniofac Surg
JT  - The Journal of craniofacial surgery
JID - 9010410
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 25038-54-4 (nylon 6)
RN  - 6879X594Z8 (Caprolactam)
RN  - 91D9GV0Z28 (Durapatite)
MH  - Absorptiometry, Photon
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - *Biomimetics
MH  - Bone Marrow Cells/*cytology
MH  - Caprolactam/*analogs & derivatives/chemistry
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Durapatite/*chemistry
MH  - Elastic Modulus
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Scanning
MH  - Nanostructures/*chemistry
MH  - Osteogenesis
MH  - Polymers/*chemistry
MH  - Porosity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skull/*surgery
MH  - *Tissue Scaffolds
EDAT- 2011/10/01 06:00
MHDA- 2011/12/30 06:00
CRDT- 2011/10/01 06:00
PHST- 2011/10/01 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2011/12/30 06:00 [medline]
AID - 00001665-201109000-00068 [pii]
AID - 10.1097/SCS.0b013e31822e8274 [doi]
PST - ppublish
SO  - J Craniofac Surg. 2011 Sep;22(5):1852-8. doi: 10.1097/SCS.0b013e31822e8274.