PMID- 21961111
OWN - NLM
STAT- MEDLINE
DCOM- 20120223
LR  - 20131121
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 136
IP  - 22
DP  - 2011 Nov 21
TI  - In situ encapsulation of laccase in nanofibers by electrospinning for development 
      of enzyme biosensors for chlorophenol monitoring.
PG  - 4802-8
LID - 10.1039/c1an15649g [doi]
AB  - A biosensor based on Trametes versicolor laccase (Lac) was developed for the 
      determination of phenolic compounds. The biosensor was prepared by in situ 
      electrospinning of a mixture of polyvinyl alcohol (PVA), Lac, PEO-PPO-PEO (F108) 
      and gold nanoparticles (Au NPs), where F108 was used as an enzyme stabilizing 
      additive and Au NPs was used to enhance the conductivity of the biosensor. Laser 
      confocal scanning microscopy and electrochemical impedance spectroscopy proved 
      that the enzyme was successfully encapsulated into the electrospun nanofibers. 
      Under the optimal conditions, the lowest detection limit was found to be 0.04 muM 
      (S/N = 3) for 2,4-DCP and the highest detection limit was found to be 12.10 muM 
      for 4-CP. The sensitivity of the biosensor obtained in the linear range for 
      chlorophenols followed the sequence 2,4-dichlorophenol (2,4-DCP) > 
      2,4,6-trichlorophenol (2,4,6-TCP) > 4-chlorophenol (4-CP). The sensing 
      performance for chlorophenols was attributed to the suitable electrochemical 
      interface of PVA/F108/Au NPs/Lac, resulting from biocompatibility, a high surface 
      area-to-volume ratio (10.42 m(2) g(-1)) and superior mechanical properties of the 
      electrospun nanofibers. The biosensor exhibited good repeatabilities of 7.6%, 
      2.8% and 9.0% (R.S.D.) and reproducibilities of 14.9%, 10.4% and 13.7% (R.S.D.) 
      for 4-CP, 2,4-DCP and 2,4,6-TCP, respectively. Lac retained 65.8% of its initial 
      activity after a 30-day storage period.
FAU - Liu, Jia
AU  - Liu J
AD  - State Key Laboratory of Water Environment Simulation, School of Environment, 
      Beijing Normal University, Beijing, P.R. China.
FAU - Niu, Junfeng
AU  - Niu J
FAU - Yin, Lifeng
AU  - Yin L
FAU - Jiang, Fan
AU  - Jiang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110929
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Capsules)
RN  - 0 (Chlorophenols)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Enzymes, Immobilized)
RN  - 059QF0KO0R (Water)
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Capsules
MH  - Chlorophenols/*analysis
MH  - Coated Materials, Biocompatible/chemistry
MH  - Dielectric Spectroscopy
MH  - Electrodes
MH  - Environmental Pollutants/*analysis
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Laccase/*chemistry/*metabolism
MH  - Nanofibers/*chemistry
MH  - Nanotechnology/*methods
MH  - Polyvinyl Alcohol/chemistry
MH  - Trametes/enzymology
MH  - Water/chemistry
EDAT- 2011/10/01 06:00
MHDA- 2012/02/24 06:00
CRDT- 2011/10/01 06:00
PHST- 2011/10/01 06:00 [entrez]
PHST- 2011/10/01 06:00 [pubmed]
PHST- 2012/02/24 06:00 [medline]
AID - 10.1039/c1an15649g [doi]
PST - ppublish
SO  - Analyst. 2011 Nov 21;136(22):4802-8. doi: 10.1039/c1an15649g. Epub 2011 Sep 29.