PMID- 21962444
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20111017
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 420
IP  - 2
DP  - 2011 Nov 25
TI  - Interaction of porcine conventional dendritic cells with swine influenza virus.
PG  - 125-34
LID - 10.1016/j.virol.2011.09.001 [doi]
AB  - Swine influenza virus (SwIV) causes sub-acute or acute respiratory infections on 
      swine farms and pigs can act as "mixing vessels" for new influenza strains. 
      Knowledge of the immune response of SwIV in its natural host, pigs, is very 
      limited. Dendritic cells (DCs) mediate the induction of immunity to pathogens, 
      but their interaction with SwIV has not been fully characterized. Thus, porcine 
      bone marrow derived DCs (poBMDCs) were exposed to a circulating strain of H3N2 
      SwIV in vitro. Infection of poBMDCs resulted in structures resembling influenza 
      virus inside poBMDCs in vesicles and also free in cytoplasm. Viral progeny was 
      undetectable in supernatant but limited replication was detected in the first 8h 
      after infection. However, viral particles from infected-poBMDCs were able to 
      induce cytopathic effect in susceptible cells only when cell-to-cell interaction 
      was favoured. The data generated in our studies reveal the particular interaction 
      of H3N2 SwIV with conventional DCs.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Mussa, Tufaria
AU  - Mussa T
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Rodriguez-Carino, Carolina
AU  - Rodriguez-Carino C
FAU - Pujol, Myriam
AU  - Pujol M
FAU - Cordoba, Lorena
AU  - Cordoba L
FAU - Busquets, Nuria
AU  - Busquets N
FAU - Crisci, Elisa
AU  - Crisci E
FAU - Dominguez, Javier
AU  - Dominguez J
FAU - Fraile, Lorenzo
AU  - Fraile L
FAU - Montoya, Maria
AU  - Montoya M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111001
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Bone Marrow Cells/virology
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Cytopathogenic Effect, Viral
MH  - Cytoplasm/virology
MH  - Dendritic Cells/cytology/*immunology/*virology
MH  - Influenza A Virus, H3N2 Subtype/*immunology/*pathogenicity
MH  - Swine
EDAT- 2011/10/04 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/10/04 06:00
PHST- 2011/05/31 00:00 [received]
PHST- 2011/06/20 00:00 [revised]
PHST- 2011/09/01 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0042-6822(11)00399-0 [pii]
AID - 10.1016/j.virol.2011.09.001 [doi]
PST - ppublish
SO  - Virology. 2011 Nov 25;420(2):125-34. doi: 10.1016/j.virol.2011.09.001. Epub 2011 
      Oct 1.