PMID- 21963292
OWN - NLM
STAT- MEDLINE
DCOM- 20121130
LR  - 20151119
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 93
IP  - 2
DP  - 2012 Oct
TI  - Detection and measurement of carbohydrate deficient transferrin in serum using 
      immuno-capture mass spectrometry: diagnostic applications for annual ryegrass 
      toxicity and corynetoxin exposure.
PG  - 611-7
LID - 10.1016/j.rvsc.2011.08.015 [doi]
AB  - The neurological livestock disease annual ryegrass toxicity (ARGT) is caused by 
      the ingestion of the naturally occurring glycolipid toxins - the corynetoxins. 
      Corynetoxins also threaten human health as potential contaminants of the food 
      supply. Presently, there are no routine diagnostic tests for 
      corynetoxins-exposure in humans or livestock. Chronic ingestion of corynetoxins 
      has been modeled in rats exposed to dietary tunicamycins for 12 months and 
      carbohydrate deficient transferrin (CDT) has been previously identified as a 
      candidate disease biomarker. Here, the technique of immuno-capture mass 
      spectrometry (icMS) was used to evaluate serum levels of CDT, discriminating 
      between control and tunicamycins-exposed rats with 85% accuracy. The icMS 
      approach is based on the combination of specific transferrin enrichment with 
      functionalized magnetic beads and automated matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). With no 
      other clinically-relevant diagnostic tests available icMS could be readily 
      adapted for high-throughput clinical assessment of corynetoxins-exposure in 
      humans or livestock.
CI  - Copyright (c) 2012. Published by Elsevier India Pvt Ltd.
FAU - Penno, M A S
AU  - Penno MA
AD  - Adelaide Proteomics Centre, University of Adelaide, Adelaide, South Australia 
      5005, Australia.
FAU - Colegate, S M
AU  - Colegate SM
FAU - Michalski, W P
AU  - Michalski WP
FAU - Hoffmann, P
AU  - Hoffmann P
LA  - eng
PT  - Journal Article
DEP - 20111001
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Biomarkers)
RN  - 0 (Carbohydrates)
RN  - 0 (Glycolipids)
RN  - 0 (Transferrin)
RN  - 0 (corynetoxins)
SB  - IM
MH  - Animals
MH  - Biomarkers/blood
MH  - Carbohydrates/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycolipids/*chemistry
MH  - Lolium/*chemistry
MH  - Rats
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Toxicity Tests, Chronic/methods/veterinary
MH  - Transferrin/chemistry/*metabolism
EDAT- 2011/10/04 06:00
MHDA- 2012/12/10 06:00
CRDT- 2011/10/04 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/08/15 00:00 [revised]
PHST- 2011/08/27 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0034-5288(11)00354-7 [pii]
AID - 10.1016/j.rvsc.2011.08.015 [doi]
PST - ppublish
SO  - Res Vet Sci. 2012 Oct;93(2):611-7. doi: 10.1016/j.rvsc.2011.08.015. Epub 2011 Oct 
      1.