PMID- 21963462
OWN - NLM
STAT- MEDLINE
DCOM- 20120325
LR  - 20111205
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 413
IP  - 1-2
DP  - 2012 Jan 18
TI  - Betaine homocysteine methyl transferase 1, a novel auto-antigen associated with 
      anti-Golgi immune reactivity.
PG  - 105-8
LID - 10.1016/j.cca.2011.09.001 [doi]
AB  - Anti-Golgi antibodies are rare autoantibodies that have been described in 
      systemic autoimmune diseases. Not all Golgi auto-antigens are known. The 
      objective of this study was to identify a novel auto-antigen associated with 
      anti-Golgi immune reactivity. Sera from a patient with Golgi immune reactivity 
      and from a control individual were used for Western blotting after 2-dimensional 
      gel separation of a rat Golgi-enriched extract. Betaine homocysteine 
      S-methyltransferase 1 (BHMT1) was identified as an auto-antigen by MALDI-TOF/TOF 
      mass spectrometry. Using human recombinant BHMT1, a strong positive blotting 
      signal was obtained with serum from the patient but not from a control. 
      Pre-absorption of the serum sample with reactivity to BHMT1 with recombinant 
      human BHMT1 resulted in decreased reactivity on Western blotting and in 
      disappearance of the Golgi-like pattern on indirect immunofluorescence. Using 
      immunocytochemistry, we confirmed the subcellular localization of BHMT1 to the 
      Golgi apparatus. Antibodies to BHMT1 were found in four of 80 samples with a 
      Golgi-pattern on indirect immunofluorescence. The antibodies were not associated 
      with a specific clinical condition. We identified BHMT1 as a novel auto-antigen 
      associated with anti-Golgi immune reactivity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Van den Bergh, Karolien
AU  - Van den Bergh K
AD  - Laboratory Medicine, Immunology, University Hospitals Leuven, Leuven, Belgium.
FAU - Vercammen, Martine
AU  - Vercammen M
FAU - Regenass, Stephan
AU  - Regenass S
FAU - Derua, Rita
AU  - Derua R
FAU - Vermeersch, Pieter
AU  - Vermeersch P
FAU - Pokreisz, Peter
AU  - Pokreisz P
FAU - Ocmant, Annick
AU  - Ocmant A
FAU - de Beeck, Katrijn Op
AU  - de Beeck KO
FAU - Janssens, Stefan
AU  - Janssens S
FAU - Waelkens, Etienne
AU  - Waelkens E
FAU - Bossuyt, Xavier
AU  - Bossuyt X
LA  - eng
PT  - Journal Article
DEP - 20110922
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Autoantigens)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.1.1.5 (Betaine-Homocysteine S-Methyltransferase)
SB  - IM
MH  - Animals
MH  - Autoantigens/*immunology
MH  - Betaine-Homocysteine S-Methyltransferase/*immunology
MH  - Blotting, Western
MH  - Cell Line
MH  - Golgi Apparatus/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Rats
MH  - Recombinant Proteins/immunology
EDAT- 2011/10/04 06:00
MHDA- 2012/03/27 06:00
CRDT- 2011/10/04 06:00
PHST- 2010/10/20 00:00 [received]
PHST- 2011/09/01 00:00 [revised]
PHST- 2011/09/01 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2012/03/27 06:00 [medline]
AID - S0009-8981(11)00485-2 [pii]
AID - 10.1016/j.cca.2011.09.001 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2012 Jan 18;413(1-2):105-8. doi: 10.1016/j.cca.2011.09.001. Epub 
      2011 Sep 22.