PMID- 21964292
OWN - NLM
STAT- MEDLINE
DCOM- 20111219
LR  - 20171116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 414
IP  - 2
DP  - 2011 Oct 22
TI  - Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the 
      activation of AMP-activated protein kinase.
PG  - 361-6
LID - 10.1016/j.bbrc.2011.09.077 [doi]
AB  - We investigated the effects of puerarin, the major isoflavone in Kudzu roots, on 
      the regulation of autophagy in ethanol-treated hepatocytes. Incubation in ethanol 
      (100 mM) for 24 h reduced cell viability by 20% and increased the cellular 
      concentrations of cholesterol and triglycerides by 40% and 20%, respectively. 
      Puerarin stimulation significantly recovered cell viability and reduced cellular 
      lipid accumulation to a level comparable to that in untreated control cells. 
      Ethanol incubation reduced autophagy significantly as assessed by 
      microtubule-associated protein1 light chain 3 (LC3) expression using 
      immunohistochemistry and immunoblot analysis. The reduced expression of LC3 was 
      restored by puerarin in a dose-dependent manner in ethanol-treated cells. The 
      effect of puerarin on mammalian targets of rapamycin (mTOR), a key regulator of 
      autophagy, was examined in ethanol-treated hepatocytes. Immunoblotting revealed 
      that puerarin significantly induced the phosphorylation of 5'AMP-activated 
      protein kinase (AMPK), thereby suppressing the mTOR target proteins S6 ribosomal 
      protein and 4E-binding protein 1. These data suggest that puerarin restored the 
      viability of cells and reduced lipid accumulation in ethanol-treated hepatocytes 
      by activating autophagy via AMPK/mTOR-mediated signaling.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Noh, Byung-Kyu
AU  - Noh BK
AD  - Division of Food Bioscience and Technology, College of Life Sciences and 
      Biotechnology, Korea University, Seoul 136-713, Republic of Korea. 
      ropang@korea.ac.kr
FAU - Lee, Jung Kyu
AU  - Lee JK
FAU - Jun, Hee-jin
AU  - Jun HJ
FAU - Lee, Ji Hae
AU  - Lee JH
FAU - Jia, Yaoyao
AU  - Jia Y
FAU - Hoang, Minh-Hien
AU  - Hoang MH
FAU - Kim, Jung-Wan
AU  - Kim JW
FAU - Park, Kwan-Hwa
AU  - Park KH
FAU - Lee, Sung-Joon
AU  - Lee SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110921
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Isoflavones)
RN  - 0 (LC3 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - AMP-Activated Protein Kinases/*biosynthesis
MH  - Animals
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Enzyme Activation
MH  - Ethanol/*pharmacology
MH  - Hepatocytes/*drug effects/enzymology
MH  - Isoflavones/*pharmacology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Rats
EDAT- 2011/10/04 06:00
MHDA- 2011/12/20 06:00
CRDT- 2011/10/04 06:00
PHST- 2011/09/13 00:00 [received]
PHST- 2011/09/14 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2011/12/20 06:00 [medline]
AID - S0006-291X(11)01681-0 [pii]
AID - 10.1016/j.bbrc.2011.09.077 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Oct 22;414(2):361-6. doi: 
      10.1016/j.bbrc.2011.09.077. Epub 2011 Sep 21.