PMID- 21965719
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20111003
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 31
IP  - 10
DP  - 2011 Oct
TI  - FISH scoring on paraffin sections versus single-cell suspension for chromophobe 
      renal carcinoma and renal oncocytoma.
PG  - 3137-42
AB  - BACKGROUND: Sectioning of the nuclei on tissue sections may give an overestimate 
      of monosomy, a feature diagnostic of chromophobe renal cell carcinoma versus 
      renal oncocytoma. The aim of the study was to assess whether or not nuclear 
      sectioning may distort the results obtained from interphase fluorescence in situ 
      hybridization (FISH) comparing the data obtained from analysis of isolated nuclei 
      derived from formalin-fixed, paraffin-embedded sections with histological 
      sections from the adjacent sections from the same tumors. PATIENTS AND METHODS: 
      Five chromophobe renal cell carcinomas and five renal oncocytomas were recruited. 
      Sections of 5 mum and 30 mum were cut for FISH to investigate chromosomes 1, 2, 6 
      10 and 17. RESULTS: FISH of isolated nuclei from renal oncocytomas showed a mean 
      increase of 3.0% for nuclei with two signals when compared to tissue sections. 
      For chromosomes 2, 6, 10 and 17, isolated nuclei showed a mean increase of 4.9% 
      of fluorescent signals over nuclei from tissue sections. FISH analysis of 
      isolated nuclei from chromophobe renal cell carcinoma showed a similar counts. 
      CONCLUSION: When a tumor section exhibits a borderline percentage of nuclei with 
      single signals around the cut-off level on tissue sections, the test should be 
      repeated on isolated nuclei to confirm chromosomal loss, diagnostic of 
      chromophobe renal carcinoma.
FAU - Brunelli, Matteo
AU  - Brunelli M
AD  - Department of Pathology and Diagnostic, University of Verona, P.le Ludovico Scuro 
      10, 37134 Verona, Italy.
FAU - Segala, Diego
AU  - Segala D
FAU - Delahunt, Brett
AU  - Delahunt B
FAU - Parolini, Claudia
AU  - Parolini C
FAU - Bersani, Samantha
AU  - Bersani S
FAU - Cheng, Liang
AU  - Cheng L
FAU - Eble, John N
AU  - Eble JN
FAU - Chilosi, Marco
AU  - Chilosi M
FAU - Gobbo, Stefano
AU  - Gobbo S
FAU - Martignoni, Guido
AU  - Martignoni G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Suspensions)
RN  - Oncocytoma, renal
SB  - IM
MH  - Adenoma, Oxyphilic/*genetics/*pathology
MH  - Carcinoma, Renal Cell/*genetics/*pathology
MH  - Cell Culture Techniques/*methods
MH  - Cell Nucleus/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Kidney Neoplasms/*genetics/*pathology
MH  - *Paraffin Embedding
MH  - Suspensions
EDAT- 2011/10/04 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/10/04 06:00
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 31/10/3137 [pii]
PST - ppublish
SO  - Anticancer Res. 2011 Oct;31(10):3137-42.