PMID- 21966447
OWN - NLM
STAT- MEDLINE
DCOM- 20120210
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 9
DP  - 2011
TI  - Temporal regulation of rapamycin on memory CTL programming by IL-12.
PG  - e25177
LID - 10.1371/journal.pone.0025177 [doi]
LID - e25177
AB  - Mammalian target of rapamycin (mTOR) is a master regulator of cell growth. Recent 
      reports have defined its important role in memory cytotoxic T lymphocyte (CTL) 
      differentiation in infections and memory programming. We report that rapamycin 
      regulated memory CTL programming by IL-12 to a similar level in a wide range of 
      concentrations, and the enhanced memory CTLs by rapamycin were functional and 
      provided similar protection against Listeria Monocytogenes challenge compared to 
      the control. In addition, rapamycin-experienced CTLs went through substantially 
      enhanced proliferation after transfer into recipients. Furthermore, the 
      regulatory function of rapamycin on CD62L expression in memory CTLs was mainly 
      contributed by the presence of rapamycin in the first 24-hr of stimulation in 
      vitro, whereas the effective window of rapamycin on the size of memory CTLs was 
      determined between 24 to 72 hrs. In conclusion, rapamycin regulates IL-12-driven 
      programming of CTLs to a similar level in a wide range of concentrations, and 
      regulates the phenotype and the size of memory CTLs in different temporal 
      windows.
FAU - Li, Xiangdong
AU  - Li X
AD  - Department of Animal and Avian Sciences, University of Maryland, College Park, 
      Maryland, United States of America.
FAU - Garcia, Karla
AU  - Garcia K
FAU - Sun, Zhifeng
AU  - Sun Z
FAU - Xiao, Zhengguo
AU  - Xiao Z
LA  - eng
PT  - Journal Article
DEP - 20110923
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 126880-86-2 (L-Selectin)
RN  - 187348-17-0 (Interleukin-12)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Immunologic Memory/drug effects/immunology
MH  - Interleukin-12/*pharmacology
MH  - L-Selectin/metabolism
MH  - Mice
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sirolimus/*pharmacology
MH  - T-Lymphocytes, Cytotoxic/*drug effects/*metabolism
PMC - PMC3179471
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/10/04 06:00
MHDA- 2012/02/11 06:00
PMCR- 2011/09/23
CRDT- 2011/10/04 06:00
PHST- 2011/06/24 00:00 [received]
PHST- 2011/08/26 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2012/02/11 06:00 [medline]
PHST- 2011/09/23 00:00 [pmc-release]
AID - PONE-D-11-11704 [pii]
AID - 10.1371/journal.pone.0025177 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(9):e25177. doi: 10.1371/journal.pone.0025177. Epub 2011 Sep 23.