PMID- 21966925
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20181201
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 12
IP  - 8
DP  - 2010 Aug
TI  - High symbiont diversity in the bone-eating worm Osedax mucofloris from shallow 
      whale-falls in the North Atlantic.
PG  - 2355-70
LID - 10.1111/j.1462-2920.2010.02299.x [doi]
AB  - Osedax worms are whale-fall specialists that infiltrate whale bones with their 
      root tissues. These are filled with endosymbiotic bacteria hypothesized to 
      provide their hosts with nutrition by extracting organic compounds from the whale 
      bones. We investigated the diversity and distribution of symbiotic bacteria in 
      Osedax mucofloris from shallow-water whale-falls in the North Atlantic using 
      comparative 16S rRNA sequence analysis and fluorescence in situ hybridization 
      (FISH). We observed a higher diversity of endosymbionts than previously described 
      from other Osedax species. Endosymbiont sequences fell into eight 
      phylogenetically distinct clusters (with 91.4-98.9% similarity between clusters), 
      and considerable microdiversity within clusters (99.5-99.7% similarity) was 
      observed. Statistical tests revealed a highly significant effect of the host 
      individual on endosymbiont diversity and distribution, with 68% of the 
      variability between clusters and 40% of the variability within clusters explained 
      by this effect. FISH analyses showed that most host individuals were dominated by 
      endosymbionts from a single cluster, with endosymbionts from less abundant 
      clusters generally confined to peripheral root tissues. The observed diversity 
      and distribution patterns indicate that the endosymbionts are transmitted 
      horizontally from the environment with repeated infection events occurring as the 
      host root tissues grow into the whale bones.
CI  - (c) 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
FAU - Verna, Caroline
AU  - Verna C
AD  - Max Planck Institute for Marine Microbiology, Celsiusstr. 1, 28359 Bremen, 
      Germany.
FAU - Ramette, Alban
AU  - Ramette A
FAU - Wiklund, Helena
AU  - Wiklund H
FAU - Dahlgren, Thomas G
AU  - Dahlgren TG
FAU - Glover, Adrian G
AU  - Glover AG
FAU - Gaill, Francoise
AU  - Gaill F
FAU - Dubilier, Nicole
AU  - Dubilier N
LA  - eng
SI  - GENBANK/FN773194
SI  - GENBANK/FN773195
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SI  - GENBANK/FN773315
PT  - Journal Article
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics
MH  - *Biodiversity
MH  - Bone and Bones
MH  - DNA, Bacterial/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - Polychaeta/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Symbiosis
MH  - Whales
EDAT- 2010/08/01 00:00
MHDA- 2011/12/14 06:00
CRDT- 2011/10/05 06:00
PHST- 2011/10/05 06:00 [entrez]
PHST- 2010/08/01 00:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - 10.1111/j.1462-2920.2010.02299.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2010 Aug;12(8):2355-70. doi: 10.1111/j.1462-2920.2010.02299.x.