PMID- 21967278
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20131121
IS  - 1742-7843 (Electronic)
IS  - 1742-7835 (Linking)
VI  - 110
IP  - 3
DP  - 2012 Mar
TI  - Dopamine D1 receptor-mediated intracellular responses in the hypothalamus after 
      co-administration of caffeine with MDMA.
PG  - 283-9
LID - 10.1111/j.1742-7843.2011.00805.x [doi]
AB  - Markers of dopamine D(1) receptor activation were determined to elucidate 
      intracellular mechanisms associated with the combined effects of caffeine and 3,4 
      methylenedioxymethamphetamine (MDMA), reported previously to produce increased 
      toxicity, when compared with either drug alone. Caffeine (10 mg/kg) and MDMA 
      (15 mg/kg) were administered to male Sprague Dawley rats alone and in 
      combination. One hour after drug administration, core body temperature and 
      phosphorylation of the dopamine D(1) -related intracellular markers, cAMP 
      response element binding protein (CREB), the dopamine and c-AMP-regulated 
      phosphoprotein of 32 kDa (DARPP-32) and expression of the immediate early gene 
      and cellular activation marker c-fos were determined in the hypothalamus. 
      Co-administration of caffeine with MDMA increased core body temperature when 
      compared with MDMA or caffeine treatment alone. Pre-treatment with the dopamine 
      D(1) receptor antagonist SCH 23390 (1 mg/kg, i.p.), 30 min. prior to caffeine and 
      MDMA administration, produced a hypothermic response to MDMA that was unaffected 
      by caffeine. Co-administration of caffeine with MDMA increased p-CREB, p-DARPP-32 
      and c-fos expression when compared with either treatment alone. Pre-treatment 
      with SCH-23390 attenuated the changes in p-CREB, p-DARPP and c-fos. The results 
      show an enhanced intracellular response when caffeine is combined with MDMA but 
      not with either agent alone suggestive of synergistic intracellular actions 
      convergent on a dopamine D(1) receptor signalling pathway. A dopamine-related 
      synergy associated with the combined administration of caffeine and MDMA may have 
      important use and safety implications for recreational drug users.
CI  - (c) 2011 The Authors. Basic & Clinical Pharmacology & Toxicology (c) 2011 Nordic 
      Pharmacological Society.
FAU - Vanattou-Saifoudine, Natacha
AU  - Vanattou-Saifoudine N
AD  - School of Pharmacy and Pharmaceutical Sciences & Trinity College Institute of 
      Neuroscience, Trinity College, Dublin, Ireland.
FAU - Behan, Brendan
AU  - Behan B
FAU - Harkin, Andrew
AU  - Harkin A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111104
PL  - England
TA  - Basic Clin Pharmacol Toxicol
JT  - Basic & clinical pharmacology & toxicology
JID - 101208422
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Dopamine and cAMP-Regulated Phosphoprotein 32)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Receptors, Dopamine D1)
RN  - 3G6A5W338E (Caffeine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Body Temperature/drug effects
MH  - Caffeine/administration & dosage/*pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/genetics/metabolism
MH  - Dopamine and cAMP-Regulated Phosphoprotein 32/genetics/metabolism
MH  - Drug Synergism
MH  - Gene Expression Regulation/drug effects
MH  - Hypothalamus/drug effects/metabolism
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-fos/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Dopamine D1/*drug effects/metabolism
MH  - Signal Transduction/drug effects
EDAT- 2011/10/05 06:00
MHDA- 2012/06/15 06:00
CRDT- 2011/10/05 06:00
PHST- 2011/10/05 06:00 [entrez]
PHST- 2011/10/05 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
AID - 10.1111/j.1742-7843.2011.00805.x [doi]
PST - ppublish
SO  - Basic Clin Pharmacol Toxicol. 2012 Mar;110(3):283-9. doi: 
      10.1111/j.1742-7843.2011.00805.x. Epub 2011 Nov 4.