PMID- 21968069
OWN - NLM
STAT- MEDLINE
DCOM- 20120227
LR  - 20220316
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 26
IP  - 1
DP  - 2012 Jan
TI  - Involvement of aquaporin-7 in the cutaneous primary immune response through 
      modulation of antigen uptake and migration in dendritic cells.
PG  - 211-8
LID - 10.1096/fj.11-186627 [doi]
AB  - Dendritic cells (DCs) have the ability to present antigen and play a critical 
      role in the induction of the acquired immune response. Skin DCs uptake antigen 
      and subsequently migrate to regional draining lymph nodes (LNs), where they 
      activate naive T cells. Here we show that the water/glycerol channel protein 
      aquaporin 7 (AQP7) is expressed on epidermal and dermal DCs and involved in the 
      initiation of primary immune responses. AQP7-deficient DCs showed a decreased 
      cellular uptake of low-molecular-mass compounds (fluorescein isothiocyanate and 
      Lucifer yellow) and high-molecular-mass substances (ovalbumin and dextran), 
      suggesting that AQP7 is involved in antigen uptake. AQP7-deficient DCs also 
      exhibited reduced chemokine-dependent cell migration in comparison to wild-type 
      DCs. Consistent with these in vitro results, AQP7-deficient mice demonstrated a 
      reduced accumulation of antigen-retaining DCs in the LNs after antigen 
      application to the skin, which could be attributed to decreased antigen uptake 
      and migration. Coincidentally, AQP7-deficient mice had impaired antigen-induced 
      sensitization in a contact hypersensitivity model. These observations suggested 
      that AQP7 in skin DCs is primarily involved in antigen uptake and in the 
      subsequent migration of DCs and is responsible for antigen presentation and the 
      promotion of downstream immune responses.
FAU - Hara-Chikuma, Mariko
AU  - Hara-Chikuma M
AD  - Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, 
      Japan. haramari@kuhp.kyoto-u.ac.jp
FAU - Sugiyama, Yoshinori
AU  - Sugiyama Y
FAU - Kabashima, Kenji
AU  - Kabashima K
FAU - Sohara, Eisei
AU  - Sohara E
FAU - Uchida, Shinichi
AU  - Uchida S
FAU - Sasaki, Sei
AU  - Sasaki S
FAU - Inoue, Shintaro
AU  - Inoue S
FAU - Miyachi, Yoshiki
AU  - Miyachi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111003
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Antigens)
RN  - 0 (Aqp7 protein, mouse)
RN  - 0 (Aquaporins)
RN  - 0 (Haptens)
RN  - 059QF0KO0R (Water)
RN  - 9006-59-1 (Ovalbumin)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Animals
MH  - Antigens/*metabolism
MH  - Aquaporins/genetics/*immunology/metabolism
MH  - Cell Movement/*immunology
MH  - Cells, Cultured
MH  - Chemotaxis/immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Dermatitis, Contact/immunology
MH  - Dermis/cytology/*immunology
MH  - Disease Models, Animal
MH  - Epidermal Cells
MH  - Epidermis/*immunology
MH  - Glycerol/metabolism
MH  - Haptens/immunology
MH  - Hypersensitivity/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Ovalbumin/immunology/pharmacology
MH  - Pinocytosis/immunology
MH  - Water/metabolism
EDAT- 2011/10/05 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/10/05 06:00
PHST- 2011/10/05 06:00 [entrez]
PHST- 2011/10/05 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - fj.11-186627 [pii]
AID - 10.1096/fj.11-186627 [doi]
PST - ppublish
SO  - FASEB J. 2012 Jan;26(1):211-8. doi: 10.1096/fj.11-186627. Epub 2011 Oct 3.