PMID- 21968078
OWN - NLM
STAT- MEDLINE
DCOM- 20120411
LR  - 20111031
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 141
IP  - 1
DP  - 2011 Dec 30
TI  - Influence of glutathione S-transferase T1 donor/recipient mismatch and anti-GSTT1 
      antibodies in hepatic graft-versus-host-disease.
PG  - 140-4
LID - 10.1016/j.imlet.2011.09.005 [doi]
AB  - B cell responses to minor histocompatibility antigens (mHags) have not been 
      extensively studied after allogeneic hematopoietic stem cell transplantation 
      (HSCT). Glutathione S-transferase T1 (GSTT1) is a drug metabolizing enzyme 
      encoded by a single gene that is highly expressed in liver and kidney. Anti-GSTT1 
      antibodies have been described in the context of antibody-mediated rejection in 
      kidney and liver transplantation, due to a mismatch between donor and recipient. 
      The aim of the present study was to investigate the specific immune response 
      against GSTT1 in HSCT with production of antibodies and their influence in the 
      development of hepatic graft-versus-host-disease (GVHD). Forty patients and their 
      respective donors were included in the study. The median follow-up time was 35.6 
      months (range 0.6-76 months) and a total of 349 serum samples were tested for the 
      presence of anti-GSTT1 antibodies by ELISA test. Statistical analysis was 
      performed by defining the GSTT1 null donor/positive recipient as mismatch 
      compared with the other three genetic combinations regarded as GSTT1-matched. 
      Antibodies were found in three patients within the group of null donor/positive 
      recipient and one within the null/null group. Development of liver GVHD, 
      particularly its acute form, was highly associated with the GSTT1-mismatch 
      (P=0.0178) and with the presence of post-transplant anti-GSTT1 antibodies 
      (P=0.0076). We conclude that GSTT1 could be considered as a new mHag in hepatic 
      GVHD. The fact that three donors were parous females and the rapid production of 
      antibodies after HSCT suggests the existence in the graft of memory B-cells 
      specific for the GSTT1 antigen.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Martinez-Bravo, Maria Jose
AU  - Martinez-Bravo MJ
AD  - Service of Immunology, Instituto de Biomedicina de Sevilla (IBIS), Hospital 
      Universitario Virgen del Rocio/CSIC/Universidad de Sevilla, Sevilla, Spain.
FAU - Tallon, Inmaculada
AU  - Tallon I
FAU - Espigado, Ildefonso
AU  - Espigado I
FAU - Perez-Simon, Jose Antonio
AU  - Perez-Simon JA
FAU - Perez-Romero, Pilar
AU  - Perez-Romero P
FAU - Gracia-Ahufinger, Irene
AU  - Gracia-Ahufinger I
FAU - Aguilera, Isabel
AU  - Aguilera I
FAU - Nunez-Roldan, Antonio
AU  - Nunez-Roldan A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110924
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Immunoglobulin G)
RN  - 0 (Isoantibodies)
RN  - EC 2.5.1.- (glutathione S-transferase T1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - B-Lymphocytes/metabolism
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Follow-Up Studies
MH  - *Glutathione Transferase/genetics/immunology
MH  - Graft vs Host Disease/*immunology
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Isoantibodies/blood
MH  - Kidney/*enzymology/pathology
MH  - Liver/*enzymology/pathology
MH  - Male
MH  - Middle Aged
MH  - Transplantation, Homologous/immunology
EDAT- 2011/10/05 06:00
MHDA- 2012/04/12 06:00
CRDT- 2011/10/05 06:00
PHST- 2011/07/27 00:00 [received]
PHST- 2011/09/05 00:00 [revised]
PHST- 2011/09/19 00:00 [accepted]
PHST- 2011/10/05 06:00 [entrez]
PHST- 2011/10/05 06:00 [pubmed]
PHST- 2012/04/12 06:00 [medline]
AID - S0165-2478(11)00227-6 [pii]
AID - 10.1016/j.imlet.2011.09.005 [doi]
PST - ppublish
SO  - Immunol Lett. 2011 Dec 30;141(1):140-4. doi: 10.1016/j.imlet.2011.09.005. Epub 
      2011 Sep 24.