PMID- 21968263
OWN - NLM
STAT- MEDLINE
DCOM- 20120305
LR  - 20151119
IS  - 1878-4046 (Electronic)
IS  - 1076-6332 (Linking)
VI  - 18
IP  - 12
DP  - 2011 Dec
TI  - Transcatheter arterial infusion with heated saline changes the vascular 
      permeability of rabbit hepatic tumors.
PG  - 1569-76
LID - 10.1016/j.acra.2011.08.010 [doi]
AB  - RATIONALE AND OBJECTIVES: The vascular permeability of tumors can be changed by 
      transarterial infusion heat, but the mechanisms remain unknown. The aim of this 
      study was to analyze the underlying causes of changes in tumor vascular 
      permeability after heated perfusion via two different modes. MATERIALS AND 
      METHODS: Thirty rabbits with VX2 hepatic tumors were randomly divided into three 
      groups of 10 rabbits each. The hepatic artery was selectively catheterized via a 
      femoral approach, and unheated saline (control group) or heated saline (60 degrees C) was 
      then injected in either a continuous (transcatheter arterial continuous perfusion 
      [TACP]) or a pulsed (transcatheter arterial pulsed perfusion [TAPP]) manner. 
      Changes in vascular permeability in the tumors were assessed using the following 
      markers and methods: (1) qualitative assessment by visual estimation on digital 
      subtraction angiography performed after the heat infusion procedure on live 
      animals and quantitative assessment by spectrophotometry using Evans blue dye 
      extravasation on tumor and liver tissue after animals were sacrificed and (2) 
      kinase domain receptor or vascular endothelial growth factor (VEGF), expressed in 
      vascular endothelial cells, assessed by immunohistochemical staining, Western 
      blot analysis, and reverse transcription polymerase chain reaction. RESULTS: 
      Tumor staining increased in the TAPP group more than in the TACP group, but not 
      in the control group, assessed on digital subtraction angiography. Extracted dye 
      was higher in tumors in the TAPP group than in those in the TACP group; extracted 
      dye in both groups was higher than in the control group. Kinase domain receptor 
      protein and messenger ribonucleic acid expression were both higher in the TAPP 
      group than in the TACP and control groups. VEGF protein expression was lower in 
      the TAPP and TACP groups than in the control group, but VEGF messenger 
      ribonucleic acid expression was higher in the TACP group than in the TAPP and 
      control groups, and VEGF messenger ribonucleic acid expression was lower in the 
      TAPP group than in the control group. CONCLUSIONS: The vascular permeability of 
      rabbit VX2 tumors significantly increased after arterial pulsed heated infusion, 
      and the protein kinase domain receptor may play a key role in this increase of 
      tumor vascular permeability.
CI  - Crown Copyright A(c) 2011. Published by Elsevier Inc. All rights reserved.
FAU - Cao, Wei
AU  - Cao W
AD  - Department of Interventional Radiology, Tangdu Hospital, The Fourth Military 
      Medical University, No 1 Xinshi Road, Xi'an, 710038, Shaanxi, China. 
      cawe-001@163.com
FAU - Lu, Qiang
AU  - Lu Q
FAU - Li, Jing-Hua
AU  - Li JH
FAU - Zhou, Chang-Xi
AU  - Zhou CX
FAU - Zhu, Jia
AU  - Zhu J
FAU - Wan, Yi
AU  - Wan Y
FAU - Liu, Yu-Feng
AU  - Liu YF
LA  - eng
PT  - Journal Article
DEP - 20111002
PL  - United States
TA  - Acad Radiol
JT  - Academic radiology
JID - 9440159
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Angiography, Digital Subtraction
MH  - Animals
MH  - Blotting, Western
MH  - Capillary Permeability/*physiology
MH  - Catheterization
MH  - Extravasation of Diagnostic and Therapeutic Materials
MH  - *Hot Temperature
MH  - Immunohistochemistry
MH  - Infusions, Intra-Arterial
MH  - Liver Neoplasms, Experimental/*blood supply
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Random Allocation
MH  - Sodium Chloride/administration & dosage
MH  - Vascular Endothelial Growth Factor A/analysis
EDAT- 2011/10/05 06:00
MHDA- 2012/03/06 06:00
CRDT- 2011/10/05 06:00
PHST- 2011/03/11 00:00 [received]
PHST- 2011/08/14 00:00 [revised]
PHST- 2011/08/16 00:00 [accepted]
PHST- 2011/10/05 06:00 [entrez]
PHST- 2011/10/05 06:00 [pubmed]
PHST- 2012/03/06 06:00 [medline]
AID - S1076-6332(11)00397-7 [pii]
AID - 10.1016/j.acra.2011.08.010 [doi]
PST - ppublish
SO  - Acad Radiol. 2011 Dec;18(12):1569-76. doi: 10.1016/j.acra.2011.08.010. Epub 2011 
      Oct 2.