PMID- 21974980
OWN - NLM
STAT- MEDLINE
DCOM- 20120301
LR  - 20201209
IS  - 1879-3460 (Electronic)
IS  - 0168-1605 (Linking)
VI  - 151
IP  - 2
DP  - 2011 Dec 2
TI  - Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ 
      hybridization (FISH).
PG  - 252-6
LID - 10.1016/j.ijfoodmicro.2011.09.007 [doi]
AB  - The transmission pathways of Helicobacter pylori in humans have not been fully 
      elucidated. Research in the last decade has proposed that foodborne transmission, 
      among others, may be a plausible route of human infection. Owing to the 
      organism's fastidious growth characteristics and its ability to convert to 
      viable, yet unculturable states upon exposure to stress conditions, the detection 
      of H. pylori in foods via culture-dependent methods has been proven to be 
      laborious, difficult and in most cases unsuccessful. Hence, nucleic acid-based 
      methods have been proposed as alternative methods but, to date, only PCR-based 
      methods have been reported in the literature. In the current study, fluorescence 
      in situ hybridization (FISH) was used for the detection of H. pylori in raw, 
      bulk-tank bovine milk. After repeated milk centrifugation and washing steps, the 
      bacterial flora of raw milk was subjected to fixation and permeabilization and H. 
      pylori detection was conducted by FISH after hybridization with an H. 
      pylori-specific 16S rRNA-directed fluorescent oligonucleotide probe. Using this 
      protocol, H. pylori was detected in four out of the twenty (20%) raw milk samples 
      examined. The data presented in this manuscript indicate that FISH can serve as 
      an alternative molecular method for screening raw bovine milk for the presence of 
      H. pylori.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Angelidis, Apostolos S
AU  - Angelidis AS
AD  - Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and 
      Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 
      54124 Thessaloniki, Greece. asangel@vet.auth.gr
FAU - Tirodimos, Ilias
AU  - Tirodimos I
FAU - Bobos, Mattheos
AU  - Bobos M
FAU - Kalamaki, Mary S
AU  - Kalamaki MS
FAU - Papageorgiou, Demetrios K
AU  - Papageorgiou DK
FAU - Arvanitidou, Malamatenia
AU  - Arvanitidou M
LA  - eng
PT  - Journal Article
DEP - 20110917
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Food Contamination/*analysis
MH  - Greece
MH  - Helicobacter pylori/genetics/*isolation & purification
MH  - *In Situ Hybridization, Fluorescence
MH  - Milk/*microbiology
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2011/10/07 06:00
MHDA- 2012/03/02 06:00
CRDT- 2011/10/07 06:00
PHST- 2011/04/20 00:00 [received]
PHST- 2011/09/09 00:00 [revised]
PHST- 2011/09/11 00:00 [accepted]
PHST- 2011/10/07 06:00 [entrez]
PHST- 2011/10/07 06:00 [pubmed]
PHST- 2012/03/02 06:00 [medline]
AID - S0168-1605(11)00525-3 [pii]
AID - 10.1016/j.ijfoodmicro.2011.09.007 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2011 Dec 2;151(2):252-6. doi: 
      10.1016/j.ijfoodmicro.2011.09.007. Epub 2011 Sep 17.