PMID- 21975812
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20111006
IS  - 1976-3786 (Electronic)
IS  - 0253-6269 (Linking)
VI  - 34
IP  - 9
DP  - 2011 Sep
TI  - Simultaneous determination of eight marker compounds in the traditional herbal 
      medicine, Sipjundaebo-tang by HPLC-DAD.
PG  - 1503-8
LID - 10.1007/s12272-011-0912-7 [doi]
AB  - Sipjundaebo-tang, known as a traditional herbal medicine, has been used in the 
      treatment of anemia, inflammation, and tumor. For simultaneous determination of 
      eight components, namely 5-HMF, paeoniflorin, ferulic acid, cinnamaldehyde, 
      decursinol, 6-gingerol, decursin, and glycyrrhizin in Sipjundaebo-tang, a high 
      performance liquid chromatography-diode array detector (HPLC-DAD) method was 
      established. In order to develop and validate this HPLC-DAD method, C(18) column 
      (S-5 mum, 4.6 x 250 mm) was used with gradient mobile phase at the column 
      temperature of 35 degrees C. The mobile phase was consisted of water and methanol 
      containing 0.1% trifluoroacetic acid. UV wavelength was set at 230, 254, 280, and 
      300 nm. Validation of the analytical method was evaluated by linearity, 
      precision, and accuracy test. Calibration curve for eight marker compounds showed 
      good linearity with R(2) > 0.9994. Limits of detection and Limits of 
      quantification ranged from 0.01 mug/mL to 0.13 mug/mL and 0.03 mug/mL to 0.41 mug/mL, 
      respectively. The relative standard deviations (RSDs) value of precision test, 
      intra-day and inter-day test, was less than 1.14% and 2.54%, respectively. The 
      results of accuracy test were varied from 98.31% to 104.88% with RSD < 2%. This 
      developed simultaneous determination method was efficient to the quality control 
      of Sipjundaebo-tang.
FAU - Yang, Hye Jin
AU  - Yang HJ
AD  - Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, 
      Kangwon National University, Chuncheon 200-701, Korea.
FAU - Ma, Jin Yeul
AU  - Ma JY
FAU - Weon, Jin Bae
AU  - Weon JB
FAU - Ma, Choong Je
AU  - Ma CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111006
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Plant Extracts)
RN  - 0 (sipjundaebo-tang)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - *Medicine, Korean Traditional
MH  - Molecular Structure
MH  - Plant Extracts/*chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
EDAT- 2011/10/07 06:00
MHDA- 2012/08/04 06:00
CRDT- 2011/10/07 06:00
PHST- 2010/12/13 00:00 [received]
PHST- 2011/04/12 00:00 [accepted]
PHST- 2011/03/29 00:00 [revised]
PHST- 2011/10/07 06:00 [entrez]
PHST- 2011/10/07 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
AID - 10.1007/s12272-011-0912-7 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2011 Sep;34(9):1503-8. doi: 10.1007/s12272-011-0912-7. Epub 2011 
      Oct 6.