PMID- 21978263
OWN - NLM
STAT- MEDLINE
DCOM- 20120127
LR  - 20211020
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 12
DP  - 2011 Oct 6
TI  - DC-STAMP knock-down deregulates cytokine production and T-cell stimulatory 
      capacity of LPS-matured dendritic cells.
PG  - 57
LID - 10.1186/1471-2172-12-57 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the highly specialized antigen presenting 
      cells of the immune system that play a key role in regulating immune responses. 
      DCs can efficiently initiate immune responses or induce tolerance. Due to this 
      dual function, DCs are studied in the context of immunotherapy for both cancer 
      and autoimmune diseases. Characterization of DC-specific genes, leading to better 
      understanding of DC immunobiology, will help to guide their use in clinical 
      settings. We previously identified DC-STAMP, a multi-membrane spanning protein 
      preferentially expressed by DCs. DC-STAMP resides in the endoplasmic reticulum 
      (ER) of immature DCs and translocates towards the Golgi compartment upon 
      maturation. In this study we knocked down DC-STAMP in mouse bone marrow-derived 
      DCs (mBMDCs) to determine its function. RESULTS: We demonstrate that DC-STAMP 
      knock-down mBMDCs secrete less IL-6, IL-12, TNF-alpha and IL-10 while IL-1 production 
      is enhanced. Moreover, LPS-matured DC-STAMP knock-down mBMDCs show impaired T 
      cell activation potential and induction of Th1 responses in an alloreaction. 
      CONCLUSIONS: We show that DC-STAMP plays an important role in cytokine production 
      by mBMDCs following LPS exposure. Our results reveal a novel function of DC-STAMP 
      in regulating DC-initiated immune responses.
FAU - Sanecka, Anna
AU  - Sanecka A
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
FAU - Ansems, Marleen
AU  - Ansems M
FAU - Prosser, Amy C
AU  - Prosser AC
FAU - Danielski, Katharina
AU  - Danielski K
FAU - Warner, Kathrin
AU  - Warner K
FAU - den Brok, Martijn H
AU  - den Brok MH
FAU - Jansen, Bastiaan J H
AU  - Jansen BJ
FAU - Eleveld-Trancikova, Dagmar
AU  - Eleveld-Trancikova D
FAU - Adema, Gosse J
AU  - Adema GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111006
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Cytokines)
RN  - 0 (DC-STAMP protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Bone Marrow/pathology
MH  - Cell Differentiation/genetics/immunology
MH  - Cell Line
MH  - Cytokines/genetics/immunology/*metabolism
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - Female
MH  - Gene Expression Regulation/genetics/immunology
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lipopolysaccharides/immunology/metabolism
MH  - Lymphocyte Activation/genetics
MH  - Membrane Proteins/genetics/immunology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Nerve Tissue Proteins/genetics/immunology/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - T-Lymphocytes/*metabolism/pathology
PMC - PMC3199277
EDAT- 2011/10/08 06:00
MHDA- 2012/01/28 06:00
PMCR- 2011/10/06
CRDT- 2011/10/08 06:00
PHST- 2011/07/12 00:00 [received]
PHST- 2011/10/06 00:00 [accepted]
PHST- 2011/10/08 06:00 [entrez]
PHST- 2011/10/08 06:00 [pubmed]
PHST- 2012/01/28 06:00 [medline]
PHST- 2011/10/06 00:00 [pmc-release]
AID - 1471-2172-12-57 [pii]
AID - 10.1186/1471-2172-12-57 [doi]
PST - epublish
SO  - BMC Immunol. 2011 Oct 6;12:57. doi: 10.1186/1471-2172-12-57.