PMID- 21980624
OWN - NLM
STAT- MEDLINE
DCOM- 20111219
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 33
IP  - 4
DP  - 2011 Aug
TI  - Determination of HER2 gene status by fully automated fluorescence microscopy.
PG  - 205-10
AB  - OBJECTIVE: To examine fluorescence in situ hybridization (FISH) in HER2 
      amplification in response rates to trastuzumab therapy and both taxane and 
      anthracycline-based chemotherapy regimens. STUDY DESIGN: A total of 400 tumor 
      sections were analyzed over an 8-month period. The sections were hybridized with 
      probes for the HER2 gene and chromosome 17 centromere using standard FISH methods 
      and analyzed on an automated fluorescence microscopy system. RESULTS: Reliable 
      and valid methods for identification of the patients that will respond to 
      treatment with trastuzumab are needed in order to achieve maximum therapy 
      efficacy and maintain cost efficiency. FISH-based analysis is potentially an 
      objective and reproducible approach to determination of HER2 gene status; 
      however, manual FISH counting is a laborious task and subject to inter and 
      intraobserver variability. CONCLUSION: The system described in this paper is a 
      valuable tool in providing a consistent approach to the interpretation of breast 
      tumor tissue analyzed by FISH analysis. In addition to consistency, an automated 
      system provides a record of the images produced that can be of immediate benefit 
      in multiple review of a difficult or equivocal case and long-term benefit in 
      terms of providing a permanent case record.
FAU - Kilpatrick, Michael W
AU  - Kilpatrick MW
AD  - Department of Pathology and Laboratory Medicine, Albany Medical College, Albany, 
      New York 12208, USA.
FAU - Sheehan, Christine E
AU  - Sheehan CE
FAU - Marganski, William A
AU  - Marganski WA
FAU - Tafas, Triantafyllos
AU  - Tafas T
FAU - Ross, Merrill S
AU  - Ross MS
FAU - Ross, Jeffrey S
AU  - Ross JS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*metabolism
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Microscopy, Fluorescence
MH  - Receptor, ErbB-2/genetics/*metabolism
EDAT- 2011/10/08 06:00
MHDA- 2011/12/20 06:00
CRDT- 2011/10/08 06:00
PHST- 2011/10/08 06:00 [entrez]
PHST- 2011/10/08 06:00 [pubmed]
PHST- 2011/12/20 06:00 [medline]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2011 Aug;33(4):205-10.