PMID- 21986526
OWN - NLM
STAT- MEDLINE
DCOM- 20111220
LR  - 20240329
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 414
IP  - 3
DP  - 2011 Oct 28
TI  - Role of lipid rafts in porcine reproductive and respiratory syndrome virus 
      infection in MARC-145 cells.
PG  - 545-50
LID - 10.1016/j.bbrc.2011.09.109 [doi]
AB  - Lipid rafts play an important role in the life cycle of many viruses. Cholesterol 
      is a critical structural component of lipid rafts. Although the porcine 
      reproductive and respiratory syndrome virus (PRRSV) has restricted cell tropism 
      for cells of the monocyte/macrophage lineage, a non-macrophage cell MARC-145 was 
      susceptible to PRRSV because of the expression of virus receptor CD163 on the 
      cell surface, therefore MARC-145 cells is used as model cell for PRRSV studies. 
      In order to determine if cholesterol is involved in PRRSV infection in MARC-145 
      cells, we used three pharmacological agents: methyl-beta cyclodextrin (MbetaCD), 
      mevinolin, and filipin complex to deplete cholesterol in MARC-145. Although these 
      agents act by different mechanisms, they all significantly inhibited PRRSV 
      infection. The inhibition could be prevented by addition of exogenous 
      cholesterol. Cell membrane cholesterol depletion after virus infection had no 
      effect on PRRSV production and cholesterol depletion pre-infection did not reduce 
      the virus attachment, suggesting cholesterol is involved in virus entry. Further 
      results showed that cholesterol depletion did not change expression levels of the 
      PRRSV receptor CD163 in MARC-145, had no effect on clathrin-mediated endocytosis, 
      but disturbed lipid-raft-dependent endocytosis. Collectively, these studies 
      suggest that cholesterol is critical for PRRSV entry, which is likely to be 
      mediated by a lipid-raft-dependent pathway.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Huang, Li
AU  - Huang L
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      210095, China.
FAU - Zhang, Yuan-peng
AU  - Zhang YP
FAU - Yu, Ya-ling
AU  - Yu YL
FAU - Sun, Ming-xia
AU  - Sun MX
FAU - Li, Chen
AU  - Li C
FAU - Chen, Pu-yan
AU  - Chen PY
FAU - Mao, Xiang
AU  - Mao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111002
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chelating Agents)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chelating Agents/chemistry
MH  - Cholesterol/chemistry/*physiology
MH  - *Endocytosis
MH  - Membrane Microdomains/chemistry/*physiology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - *Virus Internalization
PMC - PMC7092942
EDAT- 2011/10/12 06:00
MHDA- 2011/12/21 06:00
PMCR- 2011/10/02
CRDT- 2011/10/12 06:00
PHST- 2011/09/14 00:00 [received]
PHST- 2011/09/21 00:00 [accepted]
PHST- 2011/10/12 06:00 [entrez]
PHST- 2011/10/12 06:00 [pubmed]
PHST- 2011/12/21 06:00 [medline]
PHST- 2011/10/02 00:00 [pmc-release]
AID - S0006-291X(11)01724-4 [pii]
AID - 10.1016/j.bbrc.2011.09.109 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Oct 28;414(3):545-50. doi: 
      10.1016/j.bbrc.2011.09.109. Epub 2011 Oct 2.