PMID- 21987521
OWN - NLM
STAT- MEDLINE
DCOM- 20130730
LR  - 20130218
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 41
IP  - 3
DP  - 2013 Mar
TI  - Exclusion of the uniform tetraploid cells significantly improves specificity of 
      the urine FISH assay.
PG  - 218-25
LID - 10.1002/dc.21831 [doi]
AB  - The urine fluorescence in situ hybridization (FISH) assay (UroVysion), with the 
      current scoring criteria, has a higher sensitivity than routine cytopathology but 
      a lower specificity. Among 215 urine FISH tests we performed, 45 had associated 
      histopathology and clinical follow up. In this study, a cell with four signals 
      for each probe was classified as a uniform tetraploid cell (UTC); a presumed 
      reparative cell which is currently classified as an abnormal cell in the FDA 
      approved assay. By using the existing criteria, the tests were scored as positive 
      or negative before and after exclusion of the UTCs. Before the exclusion, 24 
      positive, 13 negative, seven false positive, and one false negative result were 
      obtained with 96% sensitivity and 65% specificity. After the exclusion, the 
      results changed to 22 positive, 19 negative, one false positive, and three false 
      negatives resulting in a 88% sensitivity of 88% and a 95% specificity; a 
      significant improvement in the specificity. We conclude that exclusion of the 
      UTCs as abnormal cells would result in a more solid performance of the FISH 
      assay.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Moatamed, Neda A
AU  - Moatamed NA
AD  - Department of Pathology and Laboratory Medicine, David Geffen School of Medicine 
      at UCLA, Los Angeles, California, USA. nmoatamed@mednet.ucla.edu
FAU - Apple, Sophia K
AU  - Apple SK
FAU - Bennett, Carol J
AU  - Bennett CJ
FAU - Aronson, William J
AU  - Aronson WJ
FAU - Klisak, Ivana
AU  - Klisak I
FAU - Shirley, Bobbi-Jo
AU  - Shirley BJ
FAU - Moatamed, Farhad
AU  - Moatamed F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111011
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/genetics/pathology/*urine
MH  - Cytodiagnosis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Regeneration
MH  - Sensitivity and Specificity
MH  - *Specimen Handling
MH  - *Tetraploidy
MH  - Urinalysis/methods
MH  - Urinary Bladder Neoplasms/genetics/pathology/*urine
MH  - Urine/cytology
EDAT- 2011/10/12 06:00
MHDA- 2013/07/31 06:00
CRDT- 2011/10/12 06:00
PHST- 2011/04/25 00:00 [received]
PHST- 2011/08/09 00:00 [accepted]
PHST- 2011/10/12 06:00 [entrez]
PHST- 2011/10/12 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
AID - 10.1002/dc.21831 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2013 Mar;41(3):218-25. doi: 10.1002/dc.21831. Epub 2011 Oct 11.