PMID- 21987830
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20130926
IS  - 1535-3699 (Electronic)
IS  - 1535-3699 (Linking)
VI  - 236
IP  - 11
DP  - 2011 Nov
TI  - Dendritic cells derived from HOXB4-immortalized hematopoietic bone marrow cells.
PG  - 1291-7
LID - 10.1258/ebm.2011.011140 [doi]
AB  - Dendritic cells (DCs) are essential for the generation and modulation of 
      cell-mediated adaptive immunity against infections. DC-based vaccination involves 
      transplantation of ex vivo-generated DCs loaded with antigen in vitro, but 
      remains limited by the number of autologous or allogeneic cells. While in vitro 
      expansion and differentiation of hematopoietic stem cells (HSCs) into DCs seems 
      to be the most viable alternative to overcome this problem, the complexity of HSC 
      expansion in vitro has posed significant limitations for clinical application. We 
      immortalized lineage-depleted murine hematopoietic bone marrow (lin(-)BM) cells 
      with HOXB4, and differentiated them into CD11c(+)MHCII(+) DCs. These cells showed 
      the typical DC phenotype and upregulated surface expression of co-stimulatory 
      molecules on stimulation with various toll-like receptor ligands. These DCs 
      efficiently presented exogenous antigen to T-cells via major histocompatibility 
      complex (MHC) I and II and viral antigen on infection. Finally, they showed 
      migratory capacity and were able to generate antigen-specific primed T-cells in 
      vivo. In summary, we provide evidence that HOXB4-transduced lin(-)BM cells can 
      serve as a viable means of generating fully functional DCs for scientific and 
      therapeutic applications.
FAU - Baru, Abdul Mannan
AU  - Baru AM
AD  - Department for Clinical Immunology and Rheumatology, Hannover Medical School, 
      Carl-Neuberg-Strasse 1, Hannover, Germany.
FAU - Krishnaswamy, Jayendra Kumar
AU  - Krishnaswamy JK
FAU - Rathinasamy, Anchana
AU  - Rathinasamy A
FAU - Scherr, Michaela
AU  - Scherr M
FAU - Eder, Matthias
AU  - Eder M
FAU - Behrens, Georg M N
AU  - Behrens GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111010
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (HOXB4 protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Ligands)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Dendritic Cells/*cytology/immunology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Homeodomain Proteins/*pharmacology
MH  - Humans
MH  - Immunity, Cellular
MH  - Ligands
MH  - Major Histocompatibility Complex
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phagocytosis
MH  - Phenotype
MH  - T-Lymphocytes/physiology
MH  - Toll-Like Receptors/metabolism
MH  - Transcription Factors/*pharmacology
EDAT- 2011/10/12 06:00
MHDA- 2012/01/12 06:00
CRDT- 2011/10/12 06:00
PHST- 2011/10/12 06:00 [entrez]
PHST- 2011/10/12 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
AID - ebm.2011.011140 [pii]
AID - 10.1258/ebm.2011.011140 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2011 Nov;236(11):1291-7. doi: 10.1258/ebm.2011.011140. 
      Epub 2011 Oct 10.