PMID- 2199100
OWN - NLM
STAT- MEDLINE
DCOM- 19900913
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 56
IP  - 2
DP  - 1990 Aug
TI  - Interleukin-6 production by tumor necrosis factor and 
      lipopolysaccharide-stimulated rat renal cells.
PG  - 271-9
AB  - Interleukin-6 (IL-6) is produced by various cell types, including monocytes, 
      fibroblasts, and endothelial cells. IL-6 has also been detected in the urine of 
      normal and renal transplant patients. Thus, the possible production of this 
      cytokine by glomeruli and mesangial cells was investigated. Rat glomeruli were 
      obtained by serial sieving of cortical homogenates of blood-free kidneys. 
      Mesangial cells were obtained from the glomeruli and cultured under standard 
      methods in RPMI 1640 medium containing 15% fetal calf serum. Glomeruli or 
      confluent monolayers cells were then incubated in RPMI 1640 for 18 hr, in the 
      presence or not of tumor necrosis factor-alpha (TNF alpha), lipopolysaccharide 
      (LPS), or platelet-activating factor (PAF). IL-6 activity was measured using the 
      IL-6-dependent cell line subclone (B 9-9) and expressed with respect to a 
      standard curve established with recombinant IL-6. Glomeruli generate IL-6 upon 
      TNF alpha (100 ng/ml) and LPS (1 microgram/ml), 11,500 +/- 3000 and 22,000 +/- 
      7500 U/ml, respectively. Nonstimulated mesangial cells produced 50 +/- 5 U/ml 
      (mean +/- SEM, n = 4) of IL-6. TNF alpha (1 ng/ml) and LPS (1 microgram/ml) 
      induced the production of 800 +/- 90 and 40,000 +/- 5000 U/ml, respectively (n = 
      4). In contrast, PAF (0.1 nM-1 microM) did not increase IL-6 production from 
      glomeruli or mesangial cells. These results demonstrate that renal cells 
      spontaneously generate minimal amounts of IL-6 and that this production is 
      significantly increased by TNF alpha or LPS. A synergy between LPS and TNF alpha 
      was induced in glomerular cells with 10 ng/ml of TNF alpha and graded 
      concentrations of LPS. Thus, the production of IL-6 by glomerular cells and its 
      modulation by other cytokines or endotoxins may play a role in the local 
      immunological processes leading to immune glomerular diseases.
FAU - Pirotzky, E
AU  - Pirotzky E
AD  - IHB Research Laboratories, Les Ulis, France.
FAU - Delattre, R M
AU  - Delattre RM
FAU - Hellegouarch, A
AU  - Hellegouarch A
FAU - Lonchampt, M O
AU  - Lonchampt MO
FAU - Aarden, L
AU  - Aarden L
FAU - Braquet, P
AU  - Braquet P
FAU - Galanaud, P
AU  - Galanaud P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Culture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Glomerular Mesangium/metabolism
MH  - Immunologic Techniques
MH  - Interleukin-6/*biosynthesis
MH  - Kidney Glomerulus/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1016/0090-1229(90)90148-j [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1990 Aug;56(2):271-9. doi: 
      10.1016/0090-1229(90)90148-j.