PMID- 21991822
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 25
IP  - 9
DP  - 2011 Sep
TI  - [Repair of radial segmental bone defects by combined angiopoietin 1 gene 
      transfected bone marrow mesenchymal stem cells and platelet-rich plasma tissue 
      engineered bone in rabbits].
PG  - 1115-9
AB  - OBJECTIVE: To investigate the osteogenesis effects of angiopoietin 1 (Ang-1) gene 
      transfected bone marrow mesenchymal stem cells (BMSCs) seeded on beta tricalcium 
      phosphate (beta-TCP) scaffolds (tissue engineered bone) with platelet-rich plasma 
      (PRP). METHODS: BMSCs were isolated from bone marrow tissue of rabbits. The Ang-1 
      gene was transfected into the BMSCs at passage 2 by lentivector, which were 
      seeded on beta-TCP scaffolds with PRP (0.5 mL) after 48 hours of transfection. 
      Bilateral radial segmental bone defects (15 mm in length) were created in 20 
      3-month-old New Zealand rabbits. Then the tissue engineered bone with the Ang-1 
      gene transfected BMSCs (experimental group) and untransfected BMSCs (control 
      group) were implanted into the defects in the right and left radius, 
      respectively. X-ray, histology, immunohistochemistry, and biomechanics 
      observations were done at 2, 4, 8, and 12 weeks after operation. RESULTS: In 
      vitro, the transfected rate was over 90% and RT-PCR showed that the Ang-1 
      expression were significantly increased after transfection. The X-ray films 
      showed that some callus formed at 4 weeks, partial bony union was observed at 8 
      weeks, and complete union at 12 weeks in experimental group; and bone union was 
      not observed at 12 weeks in control group. HE staining showed that capillary 
      appeared at 8 weeks and more capillaries were observed in new bone at 12 weeks in 
      experimental group; only a few capillaries were observed at 12 weeks in control 
      group. At 8 and 12 weeks, the microvascular density were (50.1 +/- 7.8) /mm2 and 
      (66.1 +/- 3.5)/mm2 in experimental group and were 0 and (30.3 +/- 7.2)/mm2 in 
      control group, showing significant differences between 2 groups at 12 weeks (Z = 
      -2.107, P = 0.031). Immunohistochemistry examination showed that the positive 
      cells can be found at 8 weeks in experimental group. And the biomechanical 
      analysis showed that maximum loads of experimental group were significantly 
      higher than those of control group in three-point bending test and compression 
      test at 12 weeks (P < 0.05). CONCLUSION: The tissue engineered bone with PRP and 
      Ang-1 can increase the osteogenic properties by enhancing capillary regeneration, 
      thus it can be used to repair radial segmental bone defects of rabbit.
FAU - Liu, Xudong
AU  - Liu X
AD  - Department of Orthopaedics, Sixth People's Hospital Affiliated to Shanghai 
      Jiaotong University, Shanghai 200233, PR China.
FAU - Cao, Le
AU  - Cao L
FAU - Jiang, Yao
AU  - Jiang Y
FAU - Zeng, Bingfang
AU  - Zeng B
FAU - Zhang, Changqing
AU  - Zhang C
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Angiopoietin-1)
RN  - 0 (Bone Substitutes)
SB  - IM
MH  - Angiopoietin-1/*genetics
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - *Bone Regeneration
MH  - Bone Substitutes
MH  - Bone and Bones
MH  - Cells, Cultured
MH  - Mesenchymal Stem Cells/cytology
MH  - Osteogenesis
MH  - *Platelet-Rich Plasma
MH  - Rabbits
MH  - Radius/pathology/*surgery
MH  - Tissue Engineering/*methods
MH  - *Transfection
MH  - Wound Healing
EDAT- 2011/10/14 06:00
MHDA- 2012/05/11 06:00
CRDT- 2011/10/14 06:00
PHST- 2011/10/14 06:00 [entrez]
PHST- 2011/10/14 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Sep;25(9):1115-9.