PMID- 21994477
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Print)
IS  - 1226-4512 (Linking)
VI  - 15
IP  - 4
DP  - 2011 Aug
TI  - Inhibitory Effects of Quercetin on Muscle-type of Nicotinic Acetylcholine 
      Receptor-Mediated Ion Currents Expressed in Xenopus Oocytes.
PG  - 195-201
LID - 10.4196/kjpp.2011.15.4.195 [doi]
AB  - The flavonoid quercetin is a low molecular weight compound generally found in 
      apple, gingko, tomato, onion and other red-colored fruits and vegetables. Like 
      other flavonoids, quercetin has diverse pharmacological actions. However, 
      relatively little is known about the influence of quercetin effects in the 
      regulation of ligand-gated ion channels. Previously, we reported that quercetin 
      regulates subsets of nicotinic acetylcholine receptors such as alpha3beta4, alpha7 and 
      alpha9alpha10. Presently, we investigated the effects of quercetin on muscle-type of 
      nicotinic acetylcholine receptor channel activity expressed in Xenopus oocytes 
      after injection of cRNA encoding human fetal or adult muscle-type of nicotinic 
      acetylcholine receptor subunits. Acetylcholine treatment elicited an inward peak 
      current (I(ACh)) in oocytes expressing both muscle-type of nicotinic 
      acetylcholine receptors and co-treatment of quercetin with acetylcholine 
      inhibited I(ACh). Pre-treatment of quercetin further inhibited I(ACh) in oocytes 
      expressing adult and fetal muscle-type nicotinic acetylcholine receptors. The 
      inhibition of I(ACh) by quercetin was reversible and concentration-dependent. The 
      IC(50) of quercetin was 18.9+/-1.2 microM in oocytes expressing adult muscle-type 
      nicotinic acetylcholine receptor. The inhibition of I(ACh) by quercetin was 
      voltage-independent and non-competitive. These results indicate that quercetin 
      might regulate human muscle-type nicotinic acetylcholine receptor channel 
      activity and that quercetin-mediated regulation of muscle-type nicotinic 
      acetylcholine receptor might be coupled to regulation of neuromuscular junction 
      activity.
FAU - Lee, Byung-Hwan
AU  - Lee BH
AD  - Department of Physiology, College of Veterinary Medicine and Bio/Molecular 
      Informatics Center, Konkuk University, Seoul 143-701, Korea.
FAU - Shin, Tae-Joon
AU  - Shin TJ
FAU - Hwang, Sung-Hee
AU  - Hwang SH
FAU - Choi, Sun-Hye
AU  - Choi SH
FAU - Kang, Jiyeon
AU  - Kang J
FAU - Kim, Hyeon-Joong
AU  - Kim HJ
FAU - Park, Chan-Woo
AU  - Park CW
FAU - Lee, Soo-Han
AU  - Lee SH
FAU - Nah, Seung-Yeol
AU  - Nah SY
LA  - eng
PT  - Journal Article
DEP - 20110831
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC3186920
OTO - NOTNLM
OT  - Flavonoids
OT  - Muscle-type nicotinic acetylcholine receptors
OT  - Quercetin
OT  - Xenopus oocyte
EDAT- 2011/10/14 06:00
MHDA- 2011/10/14 06:01
PMCR- 2011/08/01
CRDT- 2011/10/14 06:00
PHST- 2011/06/03 00:00 [received]
PHST- 2011/07/20 00:00 [revised]
PHST- 2011/08/02 00:00 [accepted]
PHST- 2011/10/14 06:00 [entrez]
PHST- 2011/10/14 06:00 [pubmed]
PHST- 2011/10/14 06:01 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2011.15.4.195 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2011 Aug;15(4):195-201. doi: 
      10.4196/kjpp.2011.15.4.195. Epub 2011 Aug 31.