PMID- 21998677
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20240313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 10
DP  - 2011
TI  - MYCT1-TV, a novel MYCT1 transcript, is regulated by c-Myc and may participate in 
      laryngeal carcinogenesis.
PG  - e25648
LID - 10.1371/journal.pone.0025648 [doi]
LID - e25648
AB  - BACKGROUND: MYCT1, a putative target of c-Myc, is a novel candidate tumor 
      suppressor gene cloned from laryngeal squamous cell carcinoma (LSCC). Its 
      transcriptional regulation and biological effects on LSCC have not been 
      clarified. METHODOLOGY/PRINCIPAL FINDINGS: Using RACE assay, we cloned a 1106 bp 
      transcript named Myc target 1 transcript variant 1 (MYCT1-TV) and confirmed its 
      transcriptional start site was located at 140 bp upstream of the ATG start codon 
      of MYCT1-TV. Luciferase, electrophoretic mobility shift and chromatin 
      immunoprecipitation assays confirmed c-Myc could regulate the promoter activity 
      of MYCT1-TV by specifically binding to the E-box elements within -886 to -655 bp 
      region. These results were further verified by site-directed mutagenesis and RNA 
      interference (RNAi) assays. MYCT1-TV and MYCT1 expressed lower in LSCC than those 
      in paired adjacent normal laryngeal tissues, and overexpression of MYCT1-TV and 
      MYCT1 could inhibit cell proliferation and invasion and promote apoptosis in LSCC 
      cells. CONCLUSIONS/SIGNIFICANCE: Our data indicate that MYCT1-TV, a novel MYCT1 
      transcript, is regulated by c-Myc and down-regulation of MYCT1-TV/MYCT1 could 
      contribute to LSCC development and function.
FAU - Fu, Shuang
AU  - Fu S
AD  - Department of Medical Genetics, China Medical University, Shenyang, People's 
      Republic of China.
FAU - Guo, Yan
AU  - Guo Y
FAU - Chen, Hong
AU  - Chen H
FAU - Xu, Zhen-Ming
AU  - Xu ZM
FAU - Qiu, Guang-Bin
AU  - Qiu GB
FAU - Zhong, Ming
AU  - Zhong M
FAU - Sun, Kai-Lai
AU  - Sun KL
FAU - Fu, Wei-Neng
AU  - Fu WN
LA  - eng
SI  - GENBANK/GU997693
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111005
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MYCT1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cloning, Molecular
MH  - E-Box Elements/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Silencing
MH  - *Genetic Variation
MH  - HEK293 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*genetics/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Proto-Oncogene Proteins c-myc/deficiency/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Transcription Initiation Site
PMC - PMC3187795
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/10/15 06:00
MHDA- 2012/02/14 06:00
PMCR- 2011/10/05
CRDT- 2011/10/15 06:00
PHST- 2011/08/19 00:00 [received]
PHST- 2011/09/07 00:00 [accepted]
PHST- 2011/10/15 06:00 [entrez]
PHST- 2011/10/15 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
PHST- 2011/10/05 00:00 [pmc-release]
AID - PONE-D-11-16411 [pii]
AID - 10.1371/journal.pone.0025648 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(10):e25648. doi: 10.1371/journal.pone.0025648. Epub 2011 Oct 5.