PMID- 22000441
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20191210
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 13
IP  - 10
DP  - 2011 Oct
TI  - [Effects of PI3K inhibitor LY294002 on the differentiation of mouse preadipocytes 
      and the expression of C/EBPalpha and PPARgamma].
PG  - 823-6
AB  - OBJECTIVE: This study examined the effects of PI3K inhibitor LY294002 on the 
      differentiation of mouse preadipocytes and the expression of CCAAT enhancer 
      binding protein alpha (C/EBPalpha) and peroxisome proliferation activated receptor gamma 
      (PPARgamma), in order to study the possible roles of insulin receptor substrate 
      (IRSs)/PI3K signal pathway in the differentiation of preadipocytes. METHODS: The 
      mouse 3T3-L1 cells were cultured normally and divided into experimental and 
      control groups. 3T3-L1 cells in the experimental group were treated with PI3K 
      inhibitor LY294002 (25 mumol/L) and those in the control group were treated with 
      DMSO culture medium. 3-isobutyl-1-methylxanthine (IBMX) (0.5 mmol/L), 
      dexamethasone (10-6 mol/L) and insulin (5 mug/mL) were used to induce the 
      differentiation of 3T3-L1 preadipocytes in both groups. Before culture, and 2, 4 
      and 8 days after culture, the cells were collected to detect the expression of 
      C/EBPalpha and PPARgamma by real-time PCR and Western blot assays. The lipid droplets of 
      3T3-L1 preadipocytes were observed by oil-red O staining. RESULTS: PI3K inhibitor 
      LY294002 did not affect the expression of C/EBPalpha and PPARgamma in un-induced 3T3-L1 
      preadipocytes (P>0.05), but decreased the expression of C/EBPalpha and PPARgamma during 
      the in vitro induced differentiation of 3T3-L1 preadipocytes compared with the 
      control group (P<0.05 or 0.01). The lipid droplets count was greatly reduced by 
      LY294002. CONCLUSIONS: PI3K inhibitor LY294002 can inhibit the differentiation of 
      mouse 3T3-LI preadipocytes and the expression of C/EBPalpha and PPARgamma in the 
      differentiation of 3T3-LI preadipoeytes, suggesting that IRSs/PI3K signal pathway 
      may play an important role in the differentiation of 3T3-L1 preadipocytes by 
      regulating the expression of C/EBPalpha and PPARgamma.
FAU - Gao, Jin-Zhi
AU  - Gao JZ
AD  - Department of Pediatrics, Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Zheng, Rui-Dan
AU  - Zheng RD
FAU - Wang, Cheng-Bin
AU  - Wang CB
FAU - Ying, Yan-Qin
AU  - Ying YQ
FAU - Luo, Xiao-Ping
AU  - Luo XP
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (Chromones)
RN  - 0 (Morpholines)
RN  - 0 (PPAR gamma)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*drug effects
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/analysis/*genetics
MH  - Cell Differentiation/drug effects
MH  - Chromones/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Mice
MH  - Morpholines/*pharmacology
MH  - PPAR gamma/analysis/*genetics
MH  - *Phosphoinositide-3 Kinase Inhibitors
MH  - RNA, Messenger/analysis
EDAT- 2011/10/18 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/10/18 06:00
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - 1008-8830(2011)10-0823-04 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2011 Oct;13(10):823-6.