PMID- 22000787
OWN - NLM
STAT- MEDLINE
DCOM- 20120301
LR  - 20111107
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 2
DP  - 2012 Jan
TI  - The use of BDNF to enhance the patency rate of small-diameter tissue-engineered 
      blood vessels through stem cell homing mechanisms.
PG  - 473-84
LID - 10.1016/j.biomaterials.2011.09.066 [doi]
AB  - The patency rate of small-diameter tissue-engineered blood vessels is the 
      determinant for their application in coronary artery bypass grafting. The 
      coronary artery is innervated by vagus nerves. The origin of vagus nerves is rich 
      in brain-derived neurotrophic factors (BDNF). We have investigated whether BDNF 
      could improve the patency rate of small-diameter tissue-engineered blood vessels 
      through promoting stem cell homing and paracrine activity. In vitro, we isolated 
      early and late endothelial progenitor cells (EPCs) and found BDNF could promote 
      single clone formation and paracrine function of EPCs, and could also induce the 
      proliferation, migration and differentiation of late EPCs. BDNF could enhance the 
      capturing of EPCs in parallel-plate flow chamber. Flow cytometric analysis and 
      laser-scanning confocal microscope showed BDNF could enhance the mobilization and 
      homing of C57BL/6 mouse EPCs after wire injury. Based on it, BDNF was coupled to 
      the lumen surface of the blood vessel matrix material incubated with collagen 
      through SPDP to construct BDNF-modified small-diameter tissue-engineered blood 
      vessel. The blood vessel patency rate was significantly higher than that of 
      control group 8 weeks after implantation in rats and the endothelialization level 
      was superior to control. These results demonstrate that BDNF can effectively 
      improve patency of small-diameter tissue-engineered blood vessels through stem 
      cell homing and paracrine, and it is expected to play an important role in the 
      construction of substitutes for coronary artery bypass grafting.
CI  - Copyright A(c) 2011 Elsevier Ltd. All rights reserved.
FAU - Zeng, Wen
AU  - Zeng W
AD  - Department of Anatomy, Key Lab for Biomechanics and Tissue Engineering of 
      Chongqing, Third Military Medical University, Shaping Ba District, Chongqing 
      400038, China.
FAU - Wen, Can
AU  - Wen C
FAU - Wu, Yangxiao
AU  - Wu Y
FAU - Li, Li
AU  - Li L
FAU - Zhou, Zhenhua
AU  - Zhou Z
FAU - Mi, Jianhong
AU  - Mi J
FAU - Chen, Wen
AU  - Chen W
FAU - Yang, Mingcan
AU  - Yang M
FAU - Hou, Chunli
AU  - Hou C
FAU - Sun, Jiansen
AU  - Sun J
FAU - Zhu, Chuhong
AU  - Zhu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111013
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Blood Vessels/*physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Endothelial Cells/cytology/drug effects
MH  - Hematopoietic Stem Cells/*cytology/*drug effects
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering/*methods
MH  - *Vascular Patency
EDAT- 2011/10/18 06:00
MHDA- 2012/03/02 06:00
CRDT- 2011/10/18 06:00
PHST- 2011/09/01 00:00 [received]
PHST- 2011/09/24 00:00 [accepted]
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2012/03/02 06:00 [medline]
AID - S0142-9612(11)01142-2 [pii]
AID - 10.1016/j.biomaterials.2011.09.066 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Jan;33(2):473-84. doi: 10.1016/j.biomaterials.2011.09.066. 
      Epub 2011 Oct 13.