PMID- 22001115
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20111121
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 318
IP  - 1
DP  - 2012 Jan 1
TI  - FoxA1 and glucocorticoid receptor crosstalk via histone H4K16 acetylation at a 
      hormone regulated enhancer.
PG  - 61-74
LID - 10.1016/j.yexcr.2011.09.016 [doi]
AB  - The forkhead transcription factor FoxA1 participates in many gene regulatory 
      events with steroid hormone receptors, one example being the integrated mouse 
      mammary tumor virus (MMTV) promoter. Its enhancer harbors several FoxA1 binding 
      sites. FoxA1 promotes glucocorticoid receptor (GR)-DNA binding and transcription. 
      Here we analyze the regulatory capacity of GR, FoxA1 and hormone in quantitative 
      terms when reconstituted with the MMTV enhancer in Xenopus oocytes. By titrating 
      each component we demonstrate that FoxA1 is required for hormone induction at low 
      GR concentration and that FoxA1 is a potent enhancer of GR-induced transcription. 
      Conversely, specific DNA binding of FoxA1 at low intranuclear concentration is 
      highly responsive to minute levels of hormone-activated GR while increased FoxA1 
      concentration results in constitutive binding. When bound to DNA, FoxA1 induces 
      DNase I hypersensitivity, this is accompanied by increased acetylation, 
      specifically at histone H4K16. Expression of FoxA1 deletion mutants demonstrated 
      its DNA binding domain to be sufficient for DNA binding in vivo. The C-terminal 
      and N-terminal domains both contribute to chromatin remodeling while the latter 
      is more important for GR mediated transcription. Thus FoxA1 is primarily 
      responsible for the chromatin presetting while GR supports chromatin presetting 
      at low hormone concentration and transcriptional induction at high hormone 
      concentration.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Belikov, Sergey
AU  - Belikov S
AD  - Department of Cell and Molecular Biology, Karolinska Institutet, SE-17177 
      Stockholm, Sweden.
FAU - Holmqvist, Per-Henrik
AU  - Holmqvist PH
FAU - Astrand, Carolina
AU  - Astrand C
FAU - Wrange, Orjan
AU  - Wrange O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111006
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Foxa1 protein, mouse)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Hepatocyte Nuclear Factor 3-alpha)
RN  - 0 (Histones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - DNA/chemistry/genetics/metabolism
MH  - Enhancer Elements, Genetic/*genetics
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Hepatocyte Nuclear Factor 3-alpha/genetics/*metabolism
MH  - Histones/*chemistry/*metabolism
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mutation
MH  - Oocytes/metabolism/virology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Xenopus laevis
EDAT- 2011/10/18 06:00
MHDA- 2012/01/14 06:00
CRDT- 2011/10/18 06:00
PHST- 2011/07/07 00:00 [received]
PHST- 2011/09/02 00:00 [revised]
PHST- 2011/09/29 00:00 [accepted]
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
AID - S0014-4827(11)00392-2 [pii]
AID - 10.1016/j.yexcr.2011.09.016 [doi]
PST - ppublish
SO  - Exp Cell Res. 2012 Jan 1;318(1):61-74. doi: 10.1016/j.yexcr.2011.09.016. Epub 
      2011 Oct 6.