PMID- 22001545
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 179
IP  - 1
DP  - 2012 Jan
TI  - Identification of a genetically diverse sequence of porcine reproductive and 
      respiratory syndrome virus in Slovenia and the impact on the sensitivity of four 
      molecular tests.
PG  - 51-6
LID - 10.1016/j.jviromet.2011.09.019 [doi]
AB  - A total 91 serum samples and 51 pig tissue samples were collected between October 
      2009 and June 2010 from 30 herds, where a clinical picture of infection or/and 
      porcine reproductive and respiratory syndrome (PRRS) antibody-positive pigs were 
      detected. Of the 142 samples tested, 65 (45.8%) were identified as porcine 
      reproductive and respiratory syndrome virus (PRRSV) positive by a one-step 
      reverse transcription and polymerase chain reaction (RT-PCR). The sequencing 
      results of 258 nucleotides in ORF7 from 30 herds with PRRSV-positive samples 
      revealed the circulation of six genetically different strains of PRRSV, all 
      belonging to the Subtype 1 (Type I). Twenty-three (76.6%) of the thirty positive 
      herds were infected with a genetically identical cluster, with 98.9-100% 
      nucleotide identity between the herds, representing the detection of a new strain 
      of PRRSV in Europe, not published previously. From these 23 herds, positive PRRSV 
      samples were detected with gel-based RT-PCR, but all gave false-negative results 
      with two commercial real-time kits. When using a third commercial real-time kit, 
      28 (93.3%) of 30 positive samples in gel-based RT-PCR were detected as the Type 
      I, confirming that the sensitivity of this real-time kit is much greater than the 
      sensitivity of the previous two. The influence of new genetic variants of PRRSV 
      circulating in Slovenia on molecular diagnosis and the control of the infection 
      is discussed.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Toplak, I
AU  - Toplak I
AD  - University of Ljubljana, Institute of Microbiology and Parasitology, Veterinary 
      Faculty, Gerbiceva 60, 1000 Ljubljana, Slovenia. ivan.toplak@vf.uni-lj.si
FAU - Rihtaric, D
AU  - Rihtaric D
FAU - Hostnik, P
AU  - Hostnik P
FAU - Grom, J
AU  - Grom J
FAU - Stukelj, M
AU  - Stukelj M
FAU - Valencak, Z
AU  - Valencak Z
LA  - eng
SI  - GENBANK/HQ213910
SI  - GENBANK/HQ213911
SI  - GENBANK/HQ213912
SI  - GENBANK/HQ213913
SI  - GENBANK/HQ213914
SI  - GENBANK/HQ213915
SI  - GENBANK/HQ213916
SI  - GENBANK/HQ213917
SI  - GENBANK/HQ213918
SI  - GENBANK/HQ213919
SI  - GENBANK/HQ213920
SI  - GENBANK/HQ213921
SI  - GENBANK/HQ213922
SI  - GENBANK/HQ213923
SI  - GENBANK/HQ213924
SI  - GENBANK/HQ213925
SI  - GENBANK/HQ213926
SI  - GENBANK/HQ213927
SI  - GENBANK/HQ213928
SI  - GENBANK/HQ213929
SI  - GENBANK/HQ213930
SI  - GENBANK/HQ213931
SI  - GENBANK/HQ213932
SI  - GENBANK/HQ213933
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111001
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - False Negative Reactions
MH  - *Genetic Variation
MH  - Genotype
MH  - Molecular Diagnostic Techniques/*methods
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & 
      purification
MH  - RNA, Viral/genetics
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA
MH  - Slovenia
MH  - Swine
MH  - Virology/*methods
EDAT- 2011/10/18 06:00
MHDA- 2012/04/20 06:00
CRDT- 2011/10/18 06:00
PHST- 2011/04/14 00:00 [received]
PHST- 2011/09/16 00:00 [revised]
PHST- 2011/09/26 00:00 [accepted]
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - S0166-0934(11)00394-6 [pii]
AID - 10.1016/j.jviromet.2011.09.019 [doi]
PST - ppublish
SO  - J Virol Methods. 2012 Jan;179(1):51-6. doi: 10.1016/j.jviromet.2011.09.019. Epub 
      2011 Oct 1.