PMID- 22002926
OWN - NLM
STAT- MEDLINE
DCOM- 20120202
LR  - 20181201
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 5
IP  - 10
DP  - 2011 Nov
TI  - PLGA artificial nerve conduits with dental pulp cells promote facial nerve 
      regeneration.
PG  - 823-30
LID - 10.1002/term.387 [doi]
AB  - A number of recent studies have shown the effectiveness of tubulation, using 
      neural progenitor cells or Schwann cells, for promoting nerve regeneration. 
      However, the use of neural cells from other neural donor tissues has potentially 
      serious clinical complications. Therefore, we focused on dental pulp as a new 
      cell source for use in such artificial conditions. Previously, we showed that 
      silicone tubes filled with dental pulp cells (DPCs) promoted facial nerve 
      regeneration in rats. However, the use of silicone tubes requires a secondary 
      removal operation because they may give rise to chronic inflammation and pain. 
      Therefore, to avoid this procedure, a new artificial device was prepared from a 
      degradable poly-DL-lactide-co-glycolide (PLGA) tube containing DPCs, and its 
      effectiveness for repairing gaps in the facial nerves of rats was investigated. A 
      PLGA tube containing rat DPCs embedded in a collagen gel was transplanted into a 
      gap in a rat facial nerve. Five days after transplantation, the facial nerves 
      connected by the PLGA tubes containing DPCs were repaired more quickly than the 
      control nerves. The PLGA tubes were resorbed in vivo and nerve regeneration was 
      observed 2 months after the transplantation. Immunostaining showed that 
      Tuj1-positive axons were present in the regenerated nerves 2 months after 
      transplantation, and osmium-toluidine blue staining showed no mineralization of 
      the regenerated nerves in those tubes containing myelinated fibres after 9 weeks. 
      PLGA tubes filled with DPCs promoted nerve regeneration and were readily resorbed 
      in vivo.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Sasaki, Ryo
AU  - Sasaki R
AD  - Department of Oral and Maxillofacial Surgery, Tokyo Women's Medical University, 
      School of Medicine, Tokyo, Japan. sasaki@oms.twmu.ac.jp
FAU - Aoki, Shunsuke
AU  - Aoki S
FAU - Yamato, Masayuki
AU  - Yamato M
FAU - Uchiyama, Hiroto
AU  - Uchiyama H
FAU - Wada, Keiji
AU  - Wada K
FAU - Ogiuchi, Hideki
AU  - Ogiuchi H
FAU - Okano, Teruo
AU  - Okano T
FAU - Ando, Tomohiro
AU  - Ando T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110117
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Absorbable Implants
MH  - Animals
MH  - Collagen
MH  - Dental Pulp/*cytology/*transplantation
MH  - *Facial Nerve
MH  - Facial Nerve Injuries/therapy
MH  - *Lactic Acid
MH  - Male
MH  - *Nerve Regeneration
MH  - Neural Stem Cells/*cytology/*transplantation
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Schwann Cells/cytology/transplantation
MH  - Time Factors
MH  - Transplantation, Autologous
EDAT- 2011/10/18 06:00
MHDA- 2012/02/03 06:00
CRDT- 2011/10/18 06:00
PHST- 2009/11/11 00:00 [received]
PHST- 2010/10/21 00:00 [accepted]
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2012/02/03 06:00 [medline]
AID - 10.1002/term.387 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2011 Nov;5(10):823-30. doi: 10.1002/term.387. Epub 2011 
      Jan 17.