PMID- 22003119
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20130109
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 52
IP  - 12
DP  - 2011 Nov 11
TI  - Longitudinal and simultaneous imaging of retinal ganglion cells and inner retinal 
      layers in a mouse model of glaucoma induced by N-methyl-D-aspartate.
PG  - 8754-62
LID - 10.1167/iovs.10-6654 [doi]
AB  - PURPOSE: To investigate the longitudinal profile of N-methyl-D-aspartate (NMDA) 
      injection-induced damage in retinal ganglion cells (RGCs) by imaging retinal Thy 
      1-cyan fluorescent protein (CFP) expression and inner retinal layers using a 
      custom-made imaging device containing short-wavelength confocal scanning laser 
      ophthalmoscope (scSLO) and speckle noise-reduced spectral-domain optical 
      coherence tomography (SD-OCT). METHODS: Simultaneous scSLO and SD-OCT 
      examinations were performed in Thy 1-CFP mice injected with NMDA (1-20 
      nanomoles). CFP-expressing RGCs were counted using scSLO images. Ganglion cell 
      complex (GCC: retinal nerve fiber layer, ganglion cell layer, and inner plexiform 
      layer) thickness around the optic disc was measured in SD-OCT images. RESULTS: 
      The RGCs rapidly decreased 1 day after NMDA injection in a dose-dependent manner 
      (65.3%, 71.7%, 49.5%, and 27.1% of the preinjection level, 2, 5, 10, and 20 
      nanomoles, respectively) and continued to decrease slightly (to 53.7%, 44.1%, 
      28.3%, and 20.2% of the preinjection level on days 14, 2, 5, 10, and 20 
      nanomoles, respectively). In contrast, dose-dependent reduction of GCC thickness 
      was first detected 4 days after injection. The thickness further decreased to 
      84.6%, 75.7%, 76.5%, and 71.4% of the preinjection level on day 14 (2, 5, 10, and 
      20 nanomoles, respectively). CONCLUSIONS: NMDA-induced RGC damage is 
      characterized by rapid RGCs loss followed by gradual reduction in GCC thickness. 
      Simultaneous imaging of CFP expression in the RGCs and inner retinal layers 
      provides a sensitive, reliable, and new method for longitudinal evaluation of 
      progressive RGC damage in experimental models of glaucoma.
FAU - Nakano, Noriko
AU  - Nakano N
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, Kyoto, Japan.
FAU - Ikeda, Hanako Ohashi
AU  - Ikeda HO
FAU - Hangai, Masanori
AU  - Hangai M
FAU - Muraoka, Yuki
AU  - Muraoka Y
FAU - Toda, Yoshinobu
AU  - Toda Y
FAU - Kakizuka, Akira
AU  - Kakizuka A
FAU - Yoshimura, Nagahisa
AU  - Yoshimura N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111111
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cyan Fluorescent Protein)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Amacrine Cells/pathology
MH  - Animals
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Glaucoma/chemically induced/*pathology
MH  - Green Fluorescent Proteins/genetics
MH  - Longitudinal Studies
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - N-Methylaspartate/*pharmacology
MH  - Ophthalmoscopy/methods/standards
MH  - Optic Disk/*pathology
MH  - Reproducibility of Results
MH  - Retinal Ganglion Cells/*pathology
MH  - Tomography, Optical Coherence/*methods/standards
EDAT- 2011/10/18 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/10/18 06:00
PHST- 2011/10/18 06:00 [entrez]
PHST- 2011/10/18 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - iovs.10-6654 [pii]
AID - 10.1167/iovs.10-6654 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2011 Nov 11;52(12):8754-62. doi: 10.1167/iovs.10-6654.