PMID- 22004818
OWN - NLM
STAT- MEDLINE
DCOM- 20111212
LR  - 20230307
IS  - 1944-9097 (Electronic)
IS  - 0362-028X (Linking)
VI  - 74
IP  - 10
DP  - 2011 Oct
TI  - Distribution of T-2 and HT-2 toxins in milling fractions of durum wheat.
PG  - 1700-7
LID - 10.4315/0362-028X.JFP-11-149 [doi]
AB  - The effect of processing on mycotoxin content in milling fractions has been 
      investigated in 10 samples of durum wheat contaminated with T-2 and HT-2 toxins 
      at levels ranging from 97 to 5,954 mug/kg (sum of T-2 and HT-2 toxins). Either 
      naturally contaminated samples or samples artificially inoculated with Fusarium 
      sporotrichioides under field conditions were used. A method based on liquid 
      chromatography-tandem mass spectrometry coupled with immunoaffinity column 
      cleanup was validated in-house for the simultaneous analysis of both toxins in a 
      variety of matrices, including uncleaned wheat, cleaned wheat, screenings, bran, 
      red dog, fine middlings, and semolina. Mean recoveries from samples spiked with 
      T-2 and HT-2 toxins at levels of 100 mug/kg ranged from 85 to 107%, with relative 
      standard deviations (RSDs) lower than 14%. The milling process led to an increase 
      of T-2 and HT-2 toxin contents up to 13- and 5-fold in screenings and bran, 
      respectively, compared with occurrence in the uncleaned wheat; however, an 
      overall reduction of T-2 and HT-2 toxins by 54% (RSD, 20%) and 89% (RSD, 3%) was 
      observed in cleaned wheat and in semolina, respectively.
FAU - Pascale, Michelangelo
AU  - Pascale M
AD  - Institute of Sciences of Food Production (ISPA), National Research Council of 
      Italy (CNR), via G. Amendola 122/O, 70126 Bari, Italy. 
      michelangelo.pascale@ispa.cnr.it
FAU - Haidukowski, Miriam
AU  - Haidukowski M
FAU - Lattanzio, Veronica Maria Teresa
AU  - Lattanzio VM
FAU - Silvestri, Marco
AU  - Silvestri M
FAU - Ranieri, Roberto
AU  - Ranieri R
FAU - Visconti, Angelo
AU  - Visconti A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - I3FL5NM3MO (T-2 Toxin)
RN  - NC6C26RM46 (HT-2 toxin)
SB  - IM
MH  - Consumer Product Safety
MH  - Flour/analysis
MH  - Food Contamination/*analysis
MH  - Food Handling/*methods
MH  - Food-Processing Industry/methods/standards
MH  - Humans
MH  - T-2 Toxin/*analogs & derivatives/*analysis
MH  - Triticum/*chemistry
EDAT- 2011/10/19 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/10/19 06:00
PHST- 2011/10/19 06:00 [entrez]
PHST- 2011/10/19 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - S0362-028X(22)11564-4 [pii]
AID - 10.4315/0362-028X.JFP-11-149 [doi]
PST - ppublish
SO  - J Food Prot. 2011 Oct;74(10):1700-7. doi: 10.4315/0362-028X.JFP-11-149.