PMID- 22005435
OWN - NLM
STAT- MEDLINE
DCOM- 20111213
LR  - 20131121
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 106 Suppl 1
DP  - 2011 Oct
TI  - Five-week dietary exposure to dry diets alters the faecal bacterial populations 
      in the domestic cat (Felis catus).
PG  - S49-52
LID - 10.1017/S0007114511000572 [doi]
AB  - The effects of wet (canned) or dry (kibbled) diets on faecal bacterial 
      populations in the cat were investigated in eight domestic short-haired cats 
      (four males and four females; averaging 6 years of age and 3.4 kg) in a nested 
      design. The cats were fed ad libitum a commercially available wet diet (moisture 
      82.0 %, crude protein 51.7 %, fat 28.9 %, carbohydrate (CHO) 8.9 % and ash 10.6 % 
      DM) for 5 weeks. On the fifth week, individual feed intakes and faecal outputs 
      were determined. Fresh faecal samples were collected twice daily, mixed for 
      homogeneity, subsampled and stored at - 85  degrees C until analysis. The cats were then 
      switched to a commercially available dry diet (moisture 8.5 %, crude protein 33.0 
      %, fat 11.0 %, CHO 49.4 % and ash 6.6 % DM) for 5 weeks, and fresh faeces were 
      sampled as described previously. Energy intake tended to be higher in cats fed 
      dry diets (P < 0.10), but body weight was similar between the two feeding periods 
      (P>0.05). Denaturing gradient gel electrophoresis (DGGE) of bacterial 16S rRNA 
      genes amplified from DNA extracted from faeces was performed. The unweighted pair 
      group method with arithmetic mean cluster analysis of bacterial community 
      profiles using Pearson's correlation revealed diet-specific clustering when the 
      same cats were fed on either a dry or a wet diet (dissimilarity between the 
      groups, 88.6 %; P < 0.001). Subsequent cloning and sequencing of five selected 
      distinct DGGE bands indicated that members of the Pelomonas and Fusobacteriaceae 
      were influenced by a short-term change in diet format. This suggests that 5-week 
      dietary exposure is sufficient to alter gastrointestinal microflora.
FAU - Bermingham, Emma N
AU  - Bermingham EN
AD  - Food Nutrition Genomics Team, Food and Textiles Group, Agri-Food and Health 
      Section, AgResearch Grasslands, Palmerston North 4442, New Zealand. 
      emma.bermingham@agresearch.co.nz
FAU - Kittelmann, Sandra
AU  - Kittelmann S
FAU - Henderson, Gemma
AU  - Henderson G
FAU - Young, Wayne
AU  - Young W
FAU - Roy, Nicole C
AU  - Roy NC
FAU - Thomas, David G
AU  - Thomas DG
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Bacteria/classification/genetics
MH  - Cats/*microbiology
MH  - Diet/*veterinary
MH  - Feces/*microbiology
MH  - Female
MH  - Male
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Water
EDAT- 2011/10/26 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/10/19 06:00
PHST- 2011/10/19 06:00 [entrez]
PHST- 2011/10/26 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - S0007114511000572 [pii]
AID - 10.1017/S0007114511000572 [doi]
PST - ppublish
SO  - Br J Nutr. 2011 Oct;106 Suppl 1:S49-52. doi: 10.1017/S0007114511000572.