PMID- 22005927
OWN - NLM
STAT- MEDLINE
DCOM- 20120430
LR  - 20211020
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 61
IP  - 1
DP  - 2012 Jan
TI  - Curcumin inhibits ox-LDL-induced MCP-1 expression by suppressing the p38MAPK and 
      NF-kappaB pathways in rat vascular smooth muscle cells.
PG  - 61-7
LID - 10.1007/s00011-011-0389-3 [doi]
AB  - OBJECTIVE: This study was designed to identify the inhibitory effect of curcumin 
      on ox-LDL-induced monocyte chemoattractant protein-1 (MCP-1) production and 
      investigated whether the effects are mediated by mitogen-activated protein kinase 
      (MAPK) and NF-kappaB pathways in rat vascular smooth muscle cells (VSMCs). METHODS: 
      The VSMCs cells were pretreated with curcumin, before stimulation with ox-LDL. 
      The ox-LDL-induced MCP-1 expression was determined by enzyme-linked immunosorbent 
      assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). 
      Intracellular signaling was investigated by Western blot. RESULTS: The 
      concentrations of MCP-1 in cell supernatant and were upregulated by ox-LDL in a 
      dose- and time-dependent manner. Additionally, curcumin decreased the expression 
      of MCP-1 in a dose-dependent manner under treatment with ox-LDL (100 mug/ml). 
      Signal transduction studies indicated that the ox-LDL-induced MCP-1 expression in 
      VSMCs could be partly reversed by the inhibitor of p38 MAPK (SB203580) and NF-kappaB 
      (BAY11-7082), whereas the ERK inhibitor (PD98059) and the JNK inhibitor 
      (SP600125) had no effect. Western blot revealed that curcumin reduced ox-LDL- 
      induced p38 MAPK phosphorylation and nuclear NF-kappaB p65 protein at the indicated 
      concentration. CONCLUSION: Curcumin suppresses ox-LDL-induced MCP-1 expression in 
      VSMCs via the p38 MAPK and NF-kappaB pathways, which suggests that the 
      anti-inflammatory effect of curcumin is related to the down-regulation of MCP-1 
      expression and offers a new theoretical basis in the anti-inflammatory effects of 
      curcumin.
FAU - Zhong, Yi
AU  - Zhong Y
AD  - Department of Cardiology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, People's Republic of China.
FAU - Liu, Tingrong
AU  - Liu T
FAU - Guo, Zhigang
AU  - Guo Z
LA  - eng
PT  - Journal Article
DEP - 20111018
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (NF-kappa B)
RN  - 0 (oxidized low density lipoprotein)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Aorta/cytology
MH  - Cell Nucleus/metabolism
MH  - Cell Survival
MH  - Chemokine CCL2/*metabolism
MH  - Curcumin/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation
MH  - Inflammation
MH  - Lipoproteins, LDL/*metabolism
MH  - Male
MH  - Muscle, Smooth, Vascular/*cytology
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2011/10/19 06:00
MHDA- 2012/05/01 06:00
CRDT- 2011/10/19 06:00
PHST- 2011/08/07 00:00 [received]
PHST- 2011/09/21 00:00 [accepted]
PHST- 2011/10/19 06:00 [entrez]
PHST- 2011/10/19 06:00 [pubmed]
PHST- 2012/05/01 06:00 [medline]
AID - 10.1007/s00011-011-0389-3 [doi]
PST - ppublish
SO  - Inflamm Res. 2012 Jan;61(1):61-7. doi: 10.1007/s00011-011-0389-3. Epub 2011 Oct 
      18.