PMID- 22006037
OWN - NLM
STAT- MEDLINE
DCOM- 20120223
LR  - 20211020
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 19
IP  - 7
DP  - 2011 Oct
TI  - Fluorescence in situ hybridization with high-complexity repeat-free 
      oligonucleotide probes generated by massively parallel synthesis.
PG  - 901-9
LID - 10.1007/s10577-011-9245-0 [doi]
AB  - The ability to visualize specific DNA sequences, on chromosomes and in nuclei, by 
      fluorescence in situ hybridization (FISH) is fundamental to many aspects of 
      genetics, genomics and cell biology. Probe selection is currently limited by the 
      availability of DNA clones or the appropriate pool of DNA sequences for PCR 
      amplification. Here, we show that liquid-phase probe pools from sequence capture 
      technology can be adapted to generate fluorescently labelled pools of 
      oligonucleotides that are very effective as repeat-free FISH probes in mammalian 
      cells. As well as detection of small (15 kb) and larger (100 kb) specific loci in 
      both cultured cells and tissue sections, we show that complex oligonucleotide 
      pools can be used as probes to visualize features of nuclear organization. Using 
      this approach, we dramatically reveal the disposition of exons around the outside 
      of a chromosome territory core and away from the nuclear periphery.
FAU - Boyle, Shelagh
AU  - Boyle S
AD  - MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, 
      University of Edinburgh, Crewe Road, Edinburgh, EH4 2XU, UK.
FAU - Rodesch, Matthew J
AU  - Rodesch MJ
FAU - Halvensleben, Heather A
AU  - Halvensleben HA
FAU - Jeddeloh, Jeffrey A
AU  - Jeddeloh JA
FAU - Bickmore, Wendy A
AU  - Bickmore WA
LA  - eng
GR  - 249956/ERC_/European Research Council/International
GR  - MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111018
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Xanthenes)
RN  - 3301-79-9 (6-carboxyfluorescein)
RN  - 82354-19-6 (Texas red)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/*chemistry/genetics
MH  - Chromosome Painting/*methods
MH  - Chromosomes/*chemistry/genetics
MH  - DNA/analysis
MH  - DNA Probes/*biosynthesis/genetics
MH  - *Exome
MH  - Exons
MH  - Fluoresceins/analysis
MH  - Fluorescent Dyes/analysis
MH  - *Genetic Loci
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Oligonucleotide Probes/*biosynthesis/genetics
MH  - Polymerase Chain Reaction
MH  - Repetitive Sequences, Nucleic Acid
MH  - Xanthenes/analysis
PMC - PMC3210351
EDAT- 2011/10/19 06:00
MHDA- 2012/02/24 06:00
PMCR- 2011/10/18
CRDT- 2011/10/19 06:00
PHST- 2011/08/09 00:00 [received]
PHST- 2011/09/19 00:00 [accepted]
PHST- 2011/09/13 00:00 [revised]
PHST- 2011/10/19 06:00 [entrez]
PHST- 2011/10/19 06:00 [pubmed]
PHST- 2012/02/24 06:00 [medline]
PHST- 2011/10/18 00:00 [pmc-release]
AID - 9245 [pii]
AID - 10.1007/s10577-011-9245-0 [doi]
PST - ppublish
SO  - Chromosome Res. 2011 Oct;19(7):901-9. doi: 10.1007/s10577-011-9245-0. Epub 2011 
      Oct 18.