PMID- 22009222
OWN - NLM
STAT- MEDLINE
DCOM- 20120223
LR  - 20211020
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 19
IP  - 7
DP  - 2011 Oct
TI  - Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS 
      cell lines.
PG  - 857-68
LID - 10.1007/s10577-011-9239-y [doi]
AB  - Induced pluripotent stem (iPS) cells have greatly provoked people's interest due 
      to their enormous potential of clinical applications. Increasing care is taken 
      with the genetic safety of iPS cells. However, up to now, the chromosomal 
      integrity of murine iPS (miPS) cells has been largely unknown. We have observed 
      recurrent trisomy and/or Robertsonian translocation (Rb) of chromosome 14 in six 
      out of nine independent miPS cell lines from three laboratories by G-banding, 
      fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) 
      analyses, while all the miPS cell lines were derived from mouse embryonic 
      fibroblasts (MEFs) or neural precursor cells (NPCs) with a normal karyotype. The 
      miPS cells with trisomy and/or Rb of chromosome 14 showed growth advantage over 
      the miPS cells with a normal karyotype. We found a significantly higher frequency 
      of Rbs in the miPS cell lines induced with c-Myc than those without c-Myc. Our 
      findings demonstrate that miPS cell lines have the propensity for chromosomal 
      aberrations and there is an obvious correlation between the extent of chromosomal 
      aberrations in miPS cells and the transcriptional factors used for their 
      reprogramming. Therefore, our study raises awareness of the need for improvements 
      of the induction conditions of miPS cells in order to avoid the chromosomal 
      aberrations and ensure future safe applications.
FAU - Chen, Qian
AU  - Chen Q
AD  - Hefei National Laboratory for Physical Sciences at Microscale and School of Life 
      Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, 
      China.
FAU - Shi, Xiaoyun
AU  - Shi X
FAU - Rudolph, Cornelia
AU  - Rudolph C
FAU - Yu, Yong
AU  - Yu Y
FAU - Zhang, Ding
AU  - Zhang D
FAU - Zhao, Xiaoyu
AU  - Zhao X
FAU - Mai, Sabine
AU  - Mai S
FAU - Wang, Gang
AU  - Wang G
FAU - Schlegelberger, Brigitte
AU  - Schlegelberger B
FAU - Shi, Qinghua
AU  - Shi Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111019
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Myc protein, mouse)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line
MH  - Chromosome Banding
MH  - Chromosomes/chemistry/*genetics
MH  - Fibroblasts/cytology/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Induced Pluripotent Stem Cells/*cytology/drug effects/metabolism
MH  - Karyotype
MH  - Karyotyping/*methods
MH  - Mice
MH  - Neurons/cytology/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism/pharmacology
MH  - *Translocation, Genetic
MH  - *Trisomy
EDAT- 2011/10/20 06:00
MHDA- 2012/02/24 06:00
CRDT- 2011/10/20 06:00
PHST- 2011/05/06 00:00 [received]
PHST- 2011/08/25 00:00 [accepted]
PHST- 2011/08/24 00:00 [revised]
PHST- 2011/10/20 06:00 [entrez]
PHST- 2011/10/20 06:00 [pubmed]
PHST- 2012/02/24 06:00 [medline]
AID - 10.1007/s10577-011-9239-y [doi]
PST - ppublish
SO  - Chromosome Res. 2011 Oct;19(7):857-68. doi: 10.1007/s10577-011-9239-y. Epub 2011 
      Oct 19.