PMID- 22009483
OWN - NLM
STAT- MEDLINE
DCOM- 20120322
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 360
IP  - 1-2
DP  - 2012 Jan
TI  - Knockdown of zinc finger protein, X-linked (ZFX) inhibits cell proliferation and 
      induces apoptosis in human laryngeal squamous cell carcinoma.
PG  - 301-7
LID - 10.1007/s11010-011-1069-x [doi]
AB  - ZFX (zinc finger protein, X-linked) gene locus on the human X chromosome is 
      structurally similar to the zinc finger protein, Y-linked gene, which may 
      constitute the primary sex-determining signal. However, the pathological roles of 
      the dysfunction of ZFX gene in human disease such as cancer have not been 
      addressed. Here, we analyzed the expression of ZFX in human laryngeal squamous 
      cell carcinoma (LSCC) tissue specimens and found a significant up-regulation 
      compared to corresponding non-tumorous LSCC tissue. Recombinant lentivirus 
      expressing ZFX short hairpin RNA (shZFX) was constructed and infected Hep-2 human 
      LSCC cells. We found that knockdown of ZFX gene resulted in suppression of 
      proliferation and colony-forming ability of Hep-2 cells, and led to S phase cell 
      cycle arrest. In addition, down-regulation of ZFX induced a significant 
      enhancement of cell apoptosis and expression changes of apoptosis-related genes. 
      These results suggest that high expression of ZFX is associated with LSCC 
      progression and knockdown of ZFX may block tumor cell growth mainly by promoting 
      cell apoptosis.
FAU - Fang, Jugao
AU  - Fang J
AD  - Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, 
      Capital Medical University, Eastern District, Beijing, People's Republic of 
      China.
FAU - Yu, Zhenkun
AU  - Yu Z
FAU - Lian, Meng
AU  - Lian M
FAU - Ma, Hongzhi
AU  - Ma H
FAU - Tai, Jun
AU  - Tai J
FAU - Zhang, Luo
AU  - Zhang L
FAU - Han, Demin
AU  - Han D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111019
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Survivin)
RN  - 0 (zinc finger protein, X-linked)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/genetics/*metabolism
MH  - Caspase 1/metabolism
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Gene Expression
MH  - *Gene Knockdown Techniques
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Kruppel-Like Transcription Factors/*genetics/metabolism
MH  - Laryngeal Neoplasms/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA Interference
MH  - S Phase Cell Cycle Checkpoints
MH  - Survivin
EDAT- 2011/10/20 06:00
MHDA- 2012/03/23 06:00
CRDT- 2011/10/20 06:00
PHST- 2011/04/19 00:00 [received]
PHST- 2011/09/08 00:00 [accepted]
PHST- 2011/10/20 06:00 [entrez]
PHST- 2011/10/20 06:00 [pubmed]
PHST- 2012/03/23 06:00 [medline]
AID - 10.1007/s11010-011-1069-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2012 Jan;360(1-2):301-7. doi: 10.1007/s11010-011-1069-x. Epub 
      2011 Oct 19.