PMID- 22011243
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20211020
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 7-8
DP  - 2012 Apr
TI  - Gas-foaming calcium phosphate cement scaffold encapsulating human umbilical cord 
      stem cells.
PG  - 816-27
LID - 10.1089/ten.TEA.2011.0267 [doi]
AB  - Tissue engineering approaches are promising to meet the increasing need for bone 
      regeneration. Calcium phosphate cement (CPC) can be injected and self-set to form 
      a scaffold with excellent osteoconductivity. The objectives of this study were to 
      develop a macroporous CPC-chitosan-fiber construct containing alginate-fibrin 
      microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) 
      and to investigate hUCMSC release from the degrading microbeads and proliferation 
      inside the porous CPC construct. The hUCMSC-encapsulated microbeads were 
      completely wrapped inside the CPC paste, with the gas-foaming porogen creating 
      macropores in CPC to provide for access to culture media. Increasing the porogen 
      content in CPC significantly increased the cell viability, from 49% of live cells 
      in CPC with 0% porogen to 86% of live cells in CPC with 15% porogen. The 
      alginate-fibrin microbeads started to degrade and release the cells inside CPC at 
      7 days. The released cells started to proliferate inside the macroporous CPC 
      construct. The live cell number inside CPC increased from 270 cells/mm(2) at 1 
      day to 350 cells/mm(2) at 21 days. The pore volume fraction of CPC increased from 
      46.8% to 78.4% using the gas-foaming method, with macropore sizes of 
      approximately 100 to 400 mum. The strength of the CPC-chitosan-fiber scaffold at 
      15% porogen was 3.8 MPa, which approximated the reported 3.5 MPa for cancellous 
      bone. In conclusion, a novel gas-foaming macroporous CPC construct containing 
      degradable alginate-fibrin microbeads was developed that encapsulated hUCMSCs. 
      The cells had good viability while wrapped inside the porous CPC construct. The 
      degradable microbeads in CPC quickly released the cells, which proliferated over 
      time inside the porous CPC. Self-setting, strong CPC with alginate-fibrin 
      microbeads for stem cell delivery is promising for bone tissue engineering 
      applications.
FAU - Chen, Wenchuan
AU  - Chen W
AD  - Biomaterials and Tissue Engineering Division, Department of Endodontics, 
      Prosthodontics and Operative Dentistry, University of Maryland Dental School, 
      Baltimore, Maryland 21201, USA.
FAU - Zhou, Hongzhi
AU  - Zhou H
FAU - Tang, Minghui
AU  - Tang M
FAU - Weir, Michael D
AU  - Weir MD
FAU - Bao, Chongyun
AU  - Bao C
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190-09/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE17974/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-08/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111209
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Calcium Phosphates)
SB  - IM
MH  - Calcium Phosphates/*chemistry
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Stem Cells/*cytology
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/*cytology
PMC - PMC3313611
EDAT- 2011/10/21 06:00
MHDA- 2012/07/24 06:00
PMCR- 2013/04/01
CRDT- 2011/10/21 06:00
PHST- 2011/10/21 06:00 [entrez]
PHST- 2011/10/21 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2011.0267 [pii]
AID - 10.1089/ten.TEA.2011.0267 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Apr;18(7-8):816-27. doi: 10.1089/ten.TEA.2011.0267. Epub 
      2011 Dec 9.