PMID- 22012683
OWN - NLM
STAT- MEDLINE
DCOM- 20120808
LR  - 20120419
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 109
IP  - 6
DP  - 2012 Jun
TI  - Construction of BAC-based physical map and analysis of chromosome rearrangement 
      in Chinese hamster ovary cell lines.
PG  - 1357-67
LID - 10.1002/bit.24347 [doi]
AB  - Chinese hamster ovary (CHO) cells have frequently been used in biotechnology for 
      many years as a mammalian host cell platform for cloning and expressing genes of 
      interest. A detailed physical chromosomal map of the CHO DG44 cell line was 
      constructed by fluorescence in situ hybridization (FISH) imaging using randomly 
      selected 303 BAC clones as hybridization probes (BAC-FISH). The two longest 
      chromosomes were completely paired chromosomes; other chromosomes were partly 
      deleted or rearranged. The end sequences of 624 BAC clones, including 287 mapped 
      BAC clones, were analyzed and 1,119 informative BAC end sequences were obtained. 
      Among 303 mapped BAC clones, 185 clones were used for BAC-FISH analysis of CHO K1 
      chromosomes and 94 clones for primary Chinese hamster lung cells. Based on this 
      constructed physical map and end sequences, the chromosome rearrangements between 
      CHO DG44, CHO K1, and primary Chinese hamster cells were investigated. Among 20 
      CHO chromosomes, eight were conserved without large rearrangement in CHO DG44, 
      CHO K1, and primary Chinese hamster cells. This result suggested that these 
      chromosomes were stable and essential in CHO cells and supposedly conserved in 
      other CHO cell lines.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Cao, Yihua
AU  - Cao Y
AD  - Department of Biotechnology, Graduate School of Engineering, Osaka University, 
      2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Kimura, Shuichi
AU  - Kimura S
FAU - Itoi, Takayuki
AU  - Itoi T
FAU - Honda, Kohsuke
AU  - Honda K
FAU - Ohtake, Hisao
AU  - Ohtake H
FAU - Omasa, Takeshi
AU  - Omasa T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111106
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - *Chromosomes
MH  - Cricetinae
MH  - Cricetulus/*genetics
MH  - *Gene Rearrangement
MH  - In Situ Hybridization, Fluorescence
MH  - *Physical Chromosome Mapping
EDAT- 2011/10/21 06:00
MHDA- 2012/08/09 06:00
CRDT- 2011/10/21 06:00
PHST- 2011/07/11 00:00 [received]
PHST- 2011/09/25 00:00 [revised]
PHST- 2011/10/11 00:00 [accepted]
PHST- 2011/10/21 06:00 [entrez]
PHST- 2011/10/21 06:00 [pubmed]
PHST- 2012/08/09 06:00 [medline]
AID - 10.1002/bit.24347 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2012 Jun;109(6):1357-67. doi: 10.1002/bit.24347. Epub 2011 Nov 
      6.