PMID- 22016843
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231105
IS  - 2159-2543 (Print)
IS  - 2159-2543 (Linking)
VI  - 1
IP  - 1
DP  - 2011 May
TI  - Independently derived targeting of 28S rDNA by A- and D-clade R2 
      retrotransposons: Plasticity of integration mechanism.
PG  - 29-37
AB  - Restriction-like endonuclease (RLE) bearing non-LTR retrotransposons are 
      site-specific elements that integrate into the genome through a target primed 
      reverse transcription mechanism (TPRT). R2 elements have been used as a model 
      system for investigating non-LTR retrotransposon integration. We previously 
      demonstrated that R2 retrotransposons require two subunits of the element-encoded 
      multifunctional protein to integrate-one subunit bound upstream of the insertion 
      site and one bound downstream. R2 elements have been phylogenetically categorized 
      into four clades: R2-A, B, C and D, that diverged from a common ancestor more 
      than 850 million years ago. All R2 elements target the same sequence within 28S 
      rDNA. The amino-terminal domain of R2Bm, an R2-D clade element, contains a single 
      zinc finger and a Myb motif that are responsible for binding R2 protein 
      downstream of the insertion site. Target site recognition is of interest as it is 
      the first step in the integration reaction and may help elucidate evolutionary 
      history and integration mechanism. The amino-terminal domain of R2-A clade 
      members contains three zinc fingers and a Myb motif. We show here that R2Lp, an 
      R2-A clade member, uses its amino-terminal DNA binding motifs to bind upstream of 
      the insertion site. Because the R2-A and R2-D clade elements recognize 28S rDNA 
      differently, we conclude the A- and D-clades represent independent targeting 
      events to the 28S site. Our results also indicate a certain plasticity of 
      insertional mechanics exists between the two clades.
FAU - Thompson, Blaine K
AU  - Thompson BK
AD  - Department of Biology; University of Texas at Arlington; Arlington, TX USA.
FAU - Christensen, Shawn M
AU  - Christensen SM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mob Genet Elements
JT  - Mobile genetic elements
JID - 101564504
PMC - PMC3190273
EDAT- 2011/10/22 06:00
MHDA- 2011/10/22 06:01
CRDT- 2011/10/22 06:00
PHST- 2011/04/21 00:00 [received]
PHST- 2011/05/16 00:00 [revised]
PHST- 2011/05/16 00:00 [accepted]
PHST- 2011/10/22 06:00 [entrez]
PHST- 2011/10/22 06:00 [pubmed]
PHST- 2011/10/22 06:01 [medline]
AID - 2159-2543-1-1-5 [pii]
AID - 10.4161/mge.1.1.16485 [doi]
PST - ppublish
SO  - Mob Genet Elements. 2011 May;1(1):29-37. doi: 10.4161/mge.1.1.16485.