PMID- 22017370
OWN - NLM
STAT- MEDLINE
DCOM- 20120713
LR  - 20211020
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 13
IP  - 2
DP  - 2012 Feb
TI  - The role of EGF receptor ubiquitination in regulating its intracellular traffic.
PG  - 329-37
LID - 10.1111/j.1600-0854.2011.01305.x [doi]
AB  - Progression of activated EGF receptor (EGFR) through the endocytic pathway 
      regulates EGFR signaling. Here we show that a non-ubiquitinated EGFR mutant, 
      unable to bind the endosomal-sorting complex required for transport (ESCRT) 
      component, Hrs, is not efficiently targeted onto intraluminal vesicles (ILVs) of 
      multivesicular endosomes/bodies (MVBs). Moreover, ubiquitination and ESCRT 
      engagement of activated EGFR are required for EGF-stimulated ILV formation. 
      Non-ubiquitinated EGFRs enter clathrin-coated tubules emanating from MVBs and 
      show enhanced recycling to the plasma membrane, compared to wild-type EGFR.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Eden, Emily R
AU  - Eden ER
AD  - UCL Institute of Ophthalmology, University College London, 11-43 Bath Street, 
      London, EC1V 9EL, UK.
FAU - Huang, Fangtian
AU  - Huang F
FAU - Sorkin, Alexander
AU  - Sorkin A
FAU - Futter, Clare E
AU  - Futter CE
LA  - eng
GR  - R01 CA112219-01A2/CA/NCI NIH HHS/United States
GR  - 078304/WT_/Wellcome Trust/United Kingdom
GR  - G0801878/MRC_/Medical Research Council/United Kingdom
GR  - R01 CA089151/CA/NCI NIH HHS/United States
GR  - CA112219/CA/NCI NIH HHS/United States
GR  - R01 CA089151-11/CA/NCI NIH HHS/United States
GR  - CA089151/CA/NCI NIH HHS/United States
GR  - R01 CA112219/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111122
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Clathrin)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Protein Subunits)
RN  - 0 (Transferrin)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (early endosome antigen 1)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Aorta/cytology
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Clathrin/genetics/metabolism
MH  - Endosomal Sorting Complexes Required for Transport/metabolism
MH  - Endosomes/metabolism
MH  - Endothelial Cells/drug effects/metabolism
MH  - Epidermal Growth Factor/metabolism/pharmacology
MH  - ErbB Receptors/genetics/*metabolism
MH  - Humans
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Multivesicular Bodies/drug effects/metabolism/ultrastructure
MH  - Mutation/physiology
MH  - Phosphorylation/drug effects
MH  - Protein Binding/drug effects/physiology
MH  - Protein Subunits/metabolism
MH  - Protein Transport/drug effects/*physiology
MH  - Sus scrofa
MH  - Transfection
MH  - Transferrin/metabolism
MH  - Ubiquitination/*physiology
MH  - Vesicular Transport Proteins/genetics/metabolism
PMC - PMC3261333
MID - NIHMS333353
COIS- CONFLICTS OF INTEREST The authors declare that they have no conflict of interest.
EDAT- 2011/10/25 06:00
MHDA- 2012/07/14 06:00
PMCR- 2013/02/01
CRDT- 2011/10/25 06:00
PHST- 2011/08/06 00:00 [received]
PHST- 2011/10/19 00:00 [revised]
PHST- 2011/10/19 00:00 [accepted]
PHST- 2011/10/25 06:00 [entrez]
PHST- 2011/10/25 06:00 [pubmed]
PHST- 2012/07/14 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - 10.1111/j.1600-0854.2011.01305.x [doi]
PST - ppublish
SO  - Traffic. 2012 Feb;13(2):329-37. doi: 10.1111/j.1600-0854.2011.01305.x. Epub 2011 
      Nov 22.