PMID- 22018238
OWN - NLM
STAT- MEDLINE
DCOM- 20120208
LR  - 20230603
IS  - 1934-6069 (Electronic)
IS  - 1931-3128 (Print)
IS  - 1931-3128 (Linking)
VI  - 10
IP  - 4
DP  - 2011 Oct 20
TI  - Topical tenofovir, a microbicide effective against HIV, inhibits herpes simplex 
      virus-2 replication.
PG  - 379-89
LID - 10.1016/j.chom.2011.08.015 [doi]
AB  - The HIV reverse-transcriptase inhibitor, tenofovir, was recently formulated into 
      a vaginal gel for use as a microbicide. In human trials, a 1% tenofovir gel 
      inhibited HIV sexual transmission by 39% and, surprisingly, herpes simplex 
      virus-2 (HSV-2) transmission by 51%. We demonstrate that the concentration 
      achieved intravaginally with a 1% tenofovir topical gel has direct antiherpetic 
      activity. Tenofovir inhibits the replication of HSV clinical isolates in human 
      embryonic fibroblasts, keratinocytes, and organotypic epithelial 3D rafts, 
      decreases HSV replication in human lymphoid and cervicovaginal tissues ex vivo, 
      and delays HSV-induced lesions and death in topically treated HSV-infected mice. 
      The active tenofovir metabolite inhibits HSV DNA-polymerase and HIV 
      reverse-transcriptase. To exert dual antiviral effects, tenofovir requires 
      topical administration to achieve a drug concentration higher than systemic 
      levels achieved by oral treatment. These findings indicate that a single topical 
      treatment, like tenofovir, can inhibit the transmission of HIV and its 
      copathogens.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Andrei, Graciela
AU  - Andrei G
AD  - Rega Institute for Medical Research, K.U. Leuven, Minderbroedersstraat 10, B-3000 
      Leuven, Belgium.
FAU - Lisco, Andrea
AU  - Lisco A
FAU - Vanpouille, Christophe
AU  - Vanpouille C
FAU - Introini, Andrea
AU  - Introini A
FAU - Balestra, Emanuela
AU  - Balestra E
FAU - van den Oord, Joost
AU  - van den Oord J
FAU - Cihlar, Tomas
AU  - Cihlar T
FAU - Perno, Carlo-Federico
AU  - Perno CF
FAU - Snoeck, Robert
AU  - Snoeck R
FAU - Margolis, Leonid
AU  - Margolis L
FAU - Balzarini, Jan
AU  - Balzarini J
LA  - eng
SI  - GENBANK/JN415116
SI  - GENBANK/JN415117
SI  - GENBANK/JN415118
SI  - GENBANK/JN415119
SI  - GENBANK/JN415120
SI  - GENBANK/JN415121
SI  - GENBANK/JN415122
SI  - GENBANK/JN415123
SI  - GENBANK/JN415124
SI  - GENBANK/JN415125
SI  - GENBANK/JN415126
GR  - Z01 HD001416-15/ImNIH/Intramural NIH HHS/United States
GR  - ZIA HD001416/ImNIH/Intramural NIH HHS/United States
GR  - ZIA HD001416-19/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - Cell Host Microbe
JT  - Cell host & microbe
JID - 101302316
RN  - 0 (Anti-Infective Agents)
RN  - 0 (DNA, Viral)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Organophosphonates)
RN  - 0 (Viral Proteins)
RN  - 99YXE507IL (Tenofovir)
RN  - EC 2.7.1.21 (Thymidine Kinase)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.11.- (DNA polymerase, Simplexvirus)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/administration & dosage/*analogs & derivatives
MH  - Administration, Topical
MH  - Animals
MH  - Anti-Infective Agents/*administration & dosage
MH  - Cells, Cultured
MH  - DNA, Viral/chemistry/genetics
MH  - DNA-Directed DNA Polymerase
MH  - Exodeoxyribonucleases/antagonists & inhibitors
MH  - HIV Reverse Transcriptase/antagonists & inhibitors
MH  - Herpes Genitalis/prevention & control/virology
MH  - Herpesvirus 2, Human/*drug effects
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nucleic Acid Synthesis Inhibitors
MH  - Organophosphonates/*administration & dosage
MH  - Sequence Analysis, DNA
MH  - Tenofovir
MH  - Thymidine Kinase/genetics
MH  - Viral Proteins/antagonists & inhibitors/genetics
PMC - PMC3201796
MID - NIHMS328183
EDAT- 2011/10/25 06:00
MHDA- 2012/02/09 06:00
PMCR- 2012/10/20
CRDT- 2011/10/25 06:00
PHST- 2011/03/25 00:00 [received]
PHST- 2011/06/23 00:00 [revised]
PHST- 2011/08/25 00:00 [accepted]
PHST- 2011/10/25 06:00 [entrez]
PHST- 2011/10/25 06:00 [pubmed]
PHST- 2012/02/09 06:00 [medline]
PHST- 2012/10/20 00:00 [pmc-release]
AID - S1931-3128(11)00289-7 [pii]
AID - 10.1016/j.chom.2011.08.015 [doi]
PST - ppublish
SO  - Cell Host Microbe. 2011 Oct 20;10(4):379-89. doi: 10.1016/j.chom.2011.08.015.