PMID- 22019053
OWN - NLM
STAT- MEDLINE
DCOM- 20121123
LR  - 20240315
IS  - 1558-1497 (Electronic)
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 33
IP  - 5
DP  - 2012 May
TI  - Brain regional angiogenic potential at the neurovascular unit during normal 
      aging.
PG  - 1004.e1-16
LID - 10.1016/j.neurobiolaging.2011.09.022 [doi]
AB  - Given strong regional specialization of the brain, cerebral angiogenesis may be 
      regionally modified during normal aging. To test this hypothesis, expression of a 
      broad cadre of angiogenesis-associated genes was assayed at the neurovascular 
      unit (NVU) in discrete brain regions of young versus aged mice by laser capture 
      microdissection coupled to quantitative real-time polymerase chain reaction 
      (PCR). Complementary quantitative capillary density/branching studies were 
      performed as well. Effects of physical exercise were also assayed to determine if 
      age-related trends could be reversed. Additionally, gene response to hypoxia was 
      probed to highlight age-associated weaknesses in adapting to this angiogenic 
      stress. Aging impacted resting expression of angiogenesis-associated genes at the 
      NVU in a region-dependent manner. Physical exercise reversed some of these 
      age-associated gene trends, as well as positively influenced cerebral capillary 
      density/branching in a region-dependent way. Lastly, hypoxia revealed a weaker 
      angiogenic response in aged brain. These results suggest heterogeneous changes in 
      angiogenic capacity of the brain during normal aging, and imply a therapeutic 
      benefit of physical exercise that acts at the level of the NVU.
CI  - Copyright A(c) 2012 Elsevier Inc. All rights reserved.
FAU - Murugesan, Nivetha
AU  - Murugesan N
AD  - Blood-Brain Barrier Laboratory, Department of Cell Biology, University of 
      Connecticut Health Center, Farmington, CT 06030, USA.
FAU - Demarest, Tyler G
AU  - Demarest TG
FAU - Madri, Joseph A
AU  - Madri JA
FAU - Pachter, Joel S
AU  - Pachter JS
LA  - eng
GR  - R01 HL051018/HL/NHLBI NIH HHS/United States
GR  - R21 NS057241/NS/NINDS NIH HHS/United States
GR  - R01 MH054718/MH/NIMH NIH HHS/United States
GR  - R01 MH054718-04/MH/NIMH NIH HHS/United States
GR  - R01-HL51018/HL/NHLBI NIH HHS/United States
GR  - R21 NS057241-01/NS/NINDS NIH HHS/United States
GR  - P01-NS00344738/NS/NINDS NIH HHS/United States
GR  - R21-NS057241/NS/NINDS NIH HHS/United States
GR  - R0-1-MH54718/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111021
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Cerebral Arteries/*growth & development/physiology
MH  - Cerebrovascular Circulation/*physiology
MH  - Cerebrovascular Disorders/genetics/physiopathology/therapy
MH  - Disease Models, Animal
MH  - Exercise Therapy/*methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neovascularization, Physiologic/*physiology
MH  - Physical Conditioning, Animal/*methods
PMC - PMC3266473
MID - NIHMS327980
COIS- Disclosure statement The authors have no actual or potential conflicts of 
      interest. All animal treatments were reviewed and approved by the Institutional 
      Animal Care and Use Committee and were in accordance with guidelines stipulated 
      by the Animal Care and Use Guidelines of the University of Connecticut Health 
      Center and the Yale University Animal Care Committee.
EDAT- 2011/10/25 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/05/01
CRDT- 2011/10/25 06:00
PHST- 2011/05/11 00:00 [received]
PHST- 2011/08/18 00:00 [revised]
PHST- 2011/09/15 00:00 [accepted]
PHST- 2011/10/25 06:00 [entrez]
PHST- 2011/10/25 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - S0197-4580(11)00374-5 [pii]
AID - 10.1016/j.neurobiolaging.2011.09.022 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2012 May;33(5):1004.e1-16. doi: 
      10.1016/j.neurobiolaging.2011.09.022. Epub 2011 Oct 21.