PMID- 22020320
OWN - NLM
STAT- MEDLINE
DCOM- 20120406
LR  - 20111214
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 199
DP  - 2011 Dec 29
TI  - Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons 
      from apoptosis.
PG  - 515-22
LID - 10.1016/j.neuroscience.2011.09.064 [doi]
AB  - Novel approaches are required in the clinical management of peripheral nerve 
      injuries because current surgical techniques result in deficient sensory 
      recovery. Microsurgery alone fails to address extensive cell death in the dorsal 
      root ganglia (DRG), in addition to poor axonal regeneration. Incorporation of 
      cultured cells into nerve conduits may offer a novel approach in which to combine 
      nerve repair and enhance axonal regeneration with neuroprotective therapies. We 
      examined apoptotic mediator expression in rat DRG neurons following repair of a 
      10-mm sciatic nerve gap using a novel synthetic conduit made of poly 
      epsilon-caprolactone (PCL) and primed with adipose-derived stem cells (ADSC) 
      differentiated towards a Schwann cell phenotype or with primary adult Schwann 
      cells. Differentiated ADSC expressed a range of neurotrophic factors including 
      nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), 
      glial-derived neurotrophic factor (GDNF), and neurotrophin-4 (NT4). Incorporation 
      of either differentiated ADSC or Schwann cells significantly increased 
      anti-apoptotic Bcl-2 mRNA expression (P<0.001) in the DRG, while significantly 
      decreasing pro-apoptotic Bax (P<0.001) and caspase-3 mRNA (P<0.01) expression. 
      Cleaved caspase-3 protein was observed in the DRG following nerve injury which 
      was attenuated when nerve repair was performed using conduits seeded with cells. 
      Cell incorporation into conduit repair of peripheral nerves demonstrates 
      experimental promise as a novel intervention to prevent DRG neuronal loss.
CI  - Copyright (c) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Reid, A J
AU  - Reid AJ
AD  - Blond McIndoe Research Labs, Regenerative Biomedicine Group, University of 
      Manchester, UK. adam.reid@manchester.ac.uk
FAU - Sun, M
AU  - Sun M
FAU - Wiberg, M
AU  - Wiberg M
FAU - Downes, S
AU  - Downes S
FAU - Terenghi, G
AU  - Terenghi G
FAU - Kingham, P J
AU  - Kingham PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111006
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
SB  - IM
MH  - Adipose Tissue/cytology/*transplantation
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cell Differentiation/physiology
MH  - Ganglia, Spinal/*metabolism/pathology
MH  - Nerve Growth Factors/metabolism
MH  - Nerve Regeneration/*physiology
MH  - Neurons/cytology
MH  - Peripheral Nerve Injuries/surgery
MH  - Polyesters
MH  - Prostheses and Implants
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Schwann Cells/cytology/*transplantation
MH  - Sciatic Nerve/injuries/surgery
MH  - Stem Cell Transplantation/*methods
MH  - Tissue Engineering/*methods
EDAT- 2011/10/25 06:00
MHDA- 2012/04/07 06:00
CRDT- 2011/10/25 06:00
PHST- 2011/08/02 00:00 [received]
PHST- 2011/09/25 00:00 [revised]
PHST- 2011/09/28 00:00 [accepted]
PHST- 2011/10/25 06:00 [entrez]
PHST- 2011/10/25 06:00 [pubmed]
PHST- 2012/04/07 06:00 [medline]
AID - S0306-4522(11)01150-X [pii]
AID - 10.1016/j.neuroscience.2011.09.064 [doi]
PST - ppublish
SO  - Neuroscience. 2011 Dec 29;199:515-22. doi: 10.1016/j.neuroscience.2011.09.064. 
      Epub 2011 Oct 6.