PMID- 22020376
OWN - NLM
STAT- MEDLINE
DCOM- 20120503
LR  - 20131121
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 26
IP  - 1
DP  - 2012 Feb
TI  - DEDC, a new flavonoid induces apoptosis via a ROS-dependent mechanism in human 
      neuroblastoma SH-SY5Y cells.
PG  - 16-23
LID - 10.1016/j.tiv.2011.10.002 [doi]
AB  - The poor prognosis of neuroblastoma and lack of effective remedies have 
      necessitated the application of new therapeutic scheme. Over the past few years, 
      it has been found that flavonoids could exert specific cytotoxic activity towards 
      cancer cells. 2-(cis-1,2-dihydroxy-4-oxo-cyclohex-5-enyl)-5,7-dihydroxy-chromone 
      (DEDC) is a plant-derived flavonoid extracted from the aerial part of 
      Macrothelypteris torresiana. The present study investigated the cytotoxic effects 
      and underlying biochemical pathway leading to cell death on the response of DEDC 
      treatment in human neuroblastoma cells. Our results indicated that (a) DEDC 
      induced SH-SY5Y cells apoptosis by elevating reactive oxygen species (ROS) 
      generation, and ROS generation that could be quenched by the antioxidants 
      N-acetyl cystein (NAC). (b) The signal transducer and activator of transcription 
      3 (STAT3) played a crucial role in DEDC-triggered cell death. (c) Nuclear factor 
      Kappa B (NF-kappaB) was activated following exposure to DEDC, and suppressing NF-kappaB 
      pathway by pyrrolidine dithiocarbamate (PDTC, a potent NF-kappaB inhibitor) 
      significantly increased neuroblastoma cell sensitivity to the pro-apoptotic 
      effect of DEDC. Overall, this study shed light on the mechanism of action of DEDC 
      and suggested more rational approaches to investigation and therapy for this 
      childhood malignancy.
CI  - Crown Copyright (c) 2011. Published by Elsevier Ltd. All rights reserved.
FAU - Liu, Huibin
AU  - Liu H
AD  - Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei 
      Province, School of Pharmacy, Tongji Medical College, Huazhong University of 
      Science and Technology, 13# Hangkong Road, Wuhan 430030, PR China.
FAU - Jiang, Chunyang
AU  - Jiang C
FAU - Xiong, Chaomei
AU  - Xiong C
FAU - Ruan, Jinlan
AU  - Ruan J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111013
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (2-(cis-1,2-dihydroxy 4-oxo-cyclohex-5-enyl)-5,7-dihydroxychromone)
RN  - 0 (Antioxidants)
RN  - 0 (Cyclohexanones)
RN  - 0 (Flavones)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrrolidines)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Thiocarbamates)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Antioxidants/pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cyclohexanones/*pharmacology
MH  - Flavones/*pharmacology
MH  - Humans
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Neuroblastoma/metabolism
MH  - Pyrrolidines/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - STAT3 Transcription Factor/metabolism
MH  - Signal Transduction/drug effects
MH  - Thiocarbamates/pharmacology
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 2011/10/25 06:00
MHDA- 2012/05/04 06:00
CRDT- 2011/10/25 06:00
PHST- 2011/05/07 00:00 [received]
PHST- 2011/07/28 00:00 [revised]
PHST- 2011/10/05 00:00 [accepted]
PHST- 2011/10/25 06:00 [entrez]
PHST- 2011/10/25 06:00 [pubmed]
PHST- 2012/05/04 06:00 [medline]
AID - S0887-2333(11)00268-2 [pii]
AID - 10.1016/j.tiv.2011.10.002 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2012 Feb;26(1):16-23. doi: 10.1016/j.tiv.2011.10.002. Epub 2011 
      Oct 13.